VCP Polyclonal antibody

• Featured Product
• KD/KO Validated

VCP Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

34

Cat no : 10736-1-AP

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

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Validation Data Gallery

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  WB analysis using 10736-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10736-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 10736-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat lung using 10736-1-AP

  rat lung tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 10736-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 10736-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10736-1-AP (VCP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10736-1-AP

  IP Result of anti-VCP (IP:10736-1-AP, 4ug; Detection:10736-1-AP 1:1000) with HeLa cells lysate 1505ug.

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  IP experiment of mouse brain using 10736-1-AP

  IP result of anti-VCP (IP:10736-1-AP, 4ug; Detection:10736-1-AP 1:300) with mouse brain tissue lysate 2400 ug.

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10736-1-AP (VCP Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10736-1-AP (VCP Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human gliomas using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human colon cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung cancer using 10736-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10736-1-AP (VCP antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, rat lung tissue, mouse lung tissue, mouse thymus tissue, C6 cells, mouse brain tissue, rat brain tissue, SH-SY5Y cells, NIH/3T3 cells
• Positive IP detected in: HeLa cells, mouse brain tissue
• Positive IHC detected in: human gliomas tissue, human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:100-1:400

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 7 publications below
• WB: See 31 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Product Information

10736-1-AP targets VCP in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VCP fusion protein Ag1002
• Full Name: valosin-containing protein
• Calculated molecular weight: 89 kDa
• Observed molecular weight: 90-100 kDa
• GenBank accession number: BC007562
• Gene symbol: VCP
• Gene ID (NCBI): 7415
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

Publications

Species	Application	Title
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
human	WB	Sci Adv; Oncoprotein SND1 hijacks nascent MHC-I heavy chain to ER-associated degradation, leading to impaired CD8+ T cell response in tumor.; Authors - Yuan Wang
human	WB	Nat Commun; Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection.; Authors - Rommel J Gestuveo ; KD Validated
human	WB	Cell Chem Biol; Targeting non-canonical pathways as a strategy to modulate the sodium iodide symporter.; Authors - Martin L Read ; KD Validated
human	WB	Hum Mol Genet; Nucleolar stress and impaired stress granule formation contribute to C9orf72 RAN translation-induced cytotoxicity.; Authors - Zhouteng Tao
human	WB	Carbohydr Polym; Hyaluronic acid/polyethyleneimine nanoparticles loaded with copper ion and disulfiram for esophageal cancer.; Authors - Ru Xu