Anticorps Polyclonal de lapin anti-EPCAM/CD326

• Phare
• Validé par KD/KO

EPCAM/CD326 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC, chIP, ELISA

Conjugaison

Non conjugué

Publications

67

Avis (1)

N° de cat : 21050-1-AP

Synonymes

EPCAM, EGP, CO17 1A, CO 17A, Cell surface glycoprotein Trop-1

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Galerie de données de validation

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  WB analysis of A431 using 21050-1-AP

  WB result of EPCAM antibody (21050-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPCAM transfected A431 cells.

• 

  WB analysis of A431 using 21050-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21050-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 21050-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 21050-1-AP (EPCAM antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21050-1-AP (EPCAM antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21050-1-AP (EPCAM antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21050-1-AP (EPCAM Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human breast cancer using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21050-1-AP (EPCAM Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse stomach using 21050-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 21050-1-AP (EPCAM/CD326 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse lung using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse lung tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of mouse colon using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using EPCAM/CD326 antibody (21050-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of MCF-7 using 21050-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using EPCAM antibody (21050-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of MCF-7 using 21050-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using 21050-1-AP (EPCAM antibody) at dilution of 1:100 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HT-29 using 21050-1-AP

  1x10^6 HT-29 cells were surface stained with 0.5 ug EPCAM/CD326 Polyclonal antibody (21050-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.5 ug Control Antibody (blue). Cells were not fixed.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules MCF-7, tissu de côlon de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu de côlon de souris, tissu de côlon humain, tissu d'estomac de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon de souris, cellules MCF-7, tissu pulmonaire de souris
• Résultats positifs en IF-Fro: tissu de côlon de souris,
• Résultats positifs en IF/ICC: cellules MCF-7,
• Résultats positifs en cytométrie: cellules HT-29,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:500-1:2000
• Immunofluorescence (IF)-FRO: IF-FRO : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 38 publications below
• IHC: See 13 publications below
• IF: See 21 publications below
• FC: See 2 publications below
• ChIP: See 1 publications below

Informations sur le produit

21050-1-AP cible EPCAM/CD326 dans les applications de WB, IHC, IF/ICC, IF-P, IF-Fro, FC, chIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: EPCAM/CD326 Protéine recombinante Ag15393
• Nom complet: epithelial cell adhesion molecule
• Masse moléculaire calculée: 314 aa, 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC014785
• Symbole du gène: EPCAM
• Identification du gène (NCBI): 4072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)

Publications

Species	Application	Title
human	IF	Mol Cancer; m6A methylation reader IGF2BP2 activates endothelial cells to promote angiogenesis and metastasis of lung adenocarcinoma; Authors - Han Fang
mouse	IHC	Cancer Cell; Loss of Lkb1 and Pten leads to lung squamous cell carcinoma with elevated PD-L1 expression.; Authors - Chunxiao Xu
mouse	IF	Gut; Cross-talk between the gut microbiota and monocyte-like macrophages mediates an inflammatory response to promote colitis-associated tumourigenesis.; Authors - Yunben Yang
human	IF	Nat Commun; Single-cell RNA sequencing highlights the role of inflammatory cancer-associated fibroblasts in bladder urothelial carcinoma.; Authors - Zhaohui Chen
human	WB	Biosens Bioelectron; Tumor-cell detection, labeling and phenotyping with an electron-doped bifunctional signal-amplifier.; Authors - Hao Lu
human	WB	EMBO J; A stress-induced tyrosine-tRNA depletion response mediates codon-based translational repression and growth suppression.; Authors - Doowon Huh

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Andrew (Verified Customer) (07-12-2024)

Got a clean band at the desired molecular weight for EPCAM on the first attempt of using this primary antibody for Western Blot. Also, there was very little background. Overall, very happy with this product.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HGSOC Commercial Cell Line

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