Validation Data Gallery
|Positive WB detected in
|HaCaT cells, A431 cells, T-47D cells, MCF-7 cells
|Positive IHC detected in
|human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|MCF-7 cells, human colon cancer tissue, human stomach cancer tissue
|Western Blot (WB)
|WB : 1:2000-1:10000
|IHC : 1:50-1:500
|IF : 1:200-1:800
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
66316-1-Ig targets EPCAM/CD326 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human samples.
|Host / Isotype
|Mouse / IgG2a
|EPCAM/CD326 fusion protein Ag15393
|epithelial cell adhesion molecule
|Calculated molecular weight
|314 aa, 35 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Protein A purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Epithelial cell adhesion molecule (EpCAM, CD326) is a type I transmembrane glycoprotein that functions as a homophilic, epithelial-specific intercellular cell-adhesion molecule. In addition to cell adhesion, EpCAM is also involved in cellular signaling, cell migration, proliferation, and differentiation. EpCAM is highly expressed on most carcinomas and therefore of potential use as a diagnostic and prognostic marker for a variety of carcinomas, and has become a therapeutic target. EpCAM may occur in distinct forms due to glycosylation. (PMID: 20837599; 19249674; 21576002; 22647938; 12691820)
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guorong (Verified Customer) (03-31-2022)
Seems not good for ARPE-19 cells