Anticorps Polyclonal de lapin anti-EGR1

• Phare
• Validé par KD/KO

EGR1 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

19

N° de cat : 55117-1-AP

Synonymes

AT225, early growth response 1, G0S30, KROX 24, KROX24, NGFI A, TIS8, Transcription factor ETR103, Transcription factor Zif268, ZIF 268, Zinc finger protein 225, Zinc finger protein Krox 24, ZNF225

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Galerie de données de validation

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  WB analysis using 55117-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55117-1-AP (EGR1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 55117-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55117-1-AP (EGR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 55117-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 55117-1-AP (EGR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 55117-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55117-1-AP (EGR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 55117-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 55117-1-AP (EGR1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 55117-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using EGR1 antibody (55117-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 55117-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using EGR1 antibody (55117-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of MCF-7 using 55117-1-AP

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human EGR1 (55117-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules MCF-7, cellules PC-3
• Résultats positifs en IHC: tissu placentaire humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7,
• Résultats positifs en cytométrie: cellules MCF-7,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IHC: See 3 publications below
• IF: See 3 publications below

Informations sur le produit

55117-1-AP cible EGR1 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: early growth response 1
• Masse moléculaire calculée: 58 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: NM_001964
• Symbole du gène: EGR1
• Identification du gène (NCBI): 1958
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Early growth response 1 (EGR1) is a member of EGR family which are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression. The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF. Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation. Egr-1 binds to the DNA sequence 5'-CGCCCCCGC-3' (Egr-site), thereby activating transcription of target genes whose products are required for mitogenesis and differentiation. Western blotting of nuclear and cytoplasmic fractions of CNS tissue verified the cytoplasmic Egr1 as 110-kDa dimer or after denaturation as a 57-kDa version, which is reflecting its theoretical molecular weight of 57 kDa. In the nucleus Egr1 is found as 75-kDa homo- or heterodimer and as 35-kDa variant under strong denaturation.

Publications

Species	Application	Title
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
human	IF	Nat Commun; Single-cell analysis of murine fibroblasts identifies neonatal to adult switching that regulates cardiomyocyte maturation.; Authors - Yin Wang
human,mouse	WB,IHC	Oncogene; N6-methyladenosine reader YTHDF2 promotes multiple myeloma cell proliferation through EGR1/p21cip1/waf1/CDK2-Cyclin E1 axis-mediated cell cycle transition; Authors - Rui Liu
mouse	WB	Int Immunopharmacol; DDIT3 aggravates pulpitis by modulating M1 polarization through EGR1 in macrophages; Authors - Yan Wang
mouse	WB	Mol Neurobiol; Satb2 Ablation Impairs Hippocampus-Based Long-Term Spatial Memory and Short-Term Working Memory and Immediate Early Genes (IEGs)-Mediated Hippocampal Synaptic Plasticity.; Authors - Ying Li
human	WB,IF,IHC	Talanta; MnO2-DNAzyme-photosensitizer nanocomposite with AIE characteristic for cell imaging and photodynamic-gene therapy.; Authors - Xudong Wang

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