HPSE Polyclonal antibody

HPSE Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16673-1-AP

Synonyms

Endo glucoronidase, HEP, heparanase, Heparanase 1, HPA, Hpa1, HPR1, HPSE, HPSE1, HSE1

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Validation Data Gallery

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  WB analysis of DU 145 using 16673-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 16673-1-AP (HPSE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of DU 145 using 16673-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 16673-1-AP (HPSE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16673-1-AP (HPSE antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 16673-1-AP (HPSE antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16673-1-AP (HPSE antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16673-1-AP (HPSE antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney slide using 16673-1-AP (HPSE Antibody) at dilution of 1:50.

• 

  IHC staining of mouse kidney using 16673-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney slide using 16673-1-AP (HPSE Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: DU 145 cells
• Positive IHC detected in: human liver cancer tissue, human placenta tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 16673-1-AP is HPSE Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: HPSE fusion protein Ag10167
• Full Name: heparanase
• Calculated Molecular Weight: 543 aa, 61 kDa
• Observed Molecular Weight: 65 kDa, 50 kDa
• GenBank Accession Number: BC051321
• Gene Symbol: HPSE
• Gene ID (NCBI): 10855
• RRID: AB_10642842
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

HPSE(Heparanase) is also named as HEP, HPA, HPA1, HPR1, HPSE1, HSE1 and belongs to the glycosyl hydrolase 79 family. It is a endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. HPSE is essential in the disassembly of the extracellular matrix (ECM) by invading cells. It has 3 isoforms produced by alternative splicing with the molecular weight of 61 kDa, 55 kDa and 53 kDa. The full length protein has six glycosylation sites. The cleavage of the 65 kDa form leads to the generation of a linker peptide, and 8 kDa and 50 kDa products. The active form, the 8/50 kDa heterodimer, is resistant to degradation and glycosylation of the 50 kDa subunit appears to be essential for its solubility.

Publications

Species	Application	Title
human,mouse	WB,IF	Cell Rep; Viral Activation of Heparanase Drives Pathogenesis of Herpes Simplex Virus-1.; Authors - Alex M Agelidis
mouse	WB	Diabetes Res Clin Pract; Heparanase-driven inflammation from the AGEs-stimulated macrophages changes the functions of glomerular endothelial cells.; Authors - Guang Xu