Anticorps Polyclonal de lapin anti-CUL4A-Specific

• Phare
• Validé par KD/KO

CUL4A-Specific Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

24

N° de cat : 14851-1-AP

Synonymes

CUL4A, cullin 4A, CUL-4A, CUL 4A

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Galerie de données de validation

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  WB analysis using 14851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 14851-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14851-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14851-1-AP (CUL4A-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HepG2 using 14851-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human CUL4A-Specific (14851-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 9 publications below
• WB: See 23 publications below
• IHC: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

14851-1-AP cible CUL4A-Specific dans les applications de WB, IHC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: cullin 4A
• Masse moléculaire calculée: 88 kDa
• Poids moléculaire observé: 77-88 kDa
• Numéro d’acquisition GenBank: NM_003589
• Symbole du gène: CUL4A
• Identification du gène (NCBI): 8451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cullin proteins assemble a large number of RING E3 ubiquitin ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Two cullin 4 (CUL4) proteins, CUL4A (87 kDa) and CUL4B(104 kDa), have been identified. The two CUL4 sequences are 83% identical. They target certain proteins for degradation by binding protein DDB1 to form a CUL4-DDB1 ubiquitin ligase complex with DDB. They form two individual E3 ligases, DDB1-CUL4ADDB2 and DDB1-CUL4BDDB2 in this process. CUL4A appeared in both the nucleus and the cytosol, suggesting a more complex mechanism for entering the nucleus. CUL4B is localized in the nucleus and facilitates the transfer of DDB1 into the nucleus independently of DDB2. This antibody is specially against CUL4A,but not CUL4B.

Publications

Species	Application	Title
human	WB,IHC	Signal Transduct Target Ther; Targeting CRL4 suppresses chemoresistant ovarian cancer growth by inducing mitophagy; Authors - Yang Meng ; KD Validated
mouse	WB	Nat Immunol; COP9 signalosome subunit 8 is essential for peripheral T cell homeostasis and antigen receptor-induced entry into the cell cycle from quiescence.; Authors - Menon Suchithra S
mouse	WB	Mol Cell; Negative regulation of AMPK signaling by high glucose via E3 ubiquitin ligase MG53.; Authors - Peng Jiang ; KD Validated
human	WB	Dev Cell; AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.; Authors - Manuela Antonioli ; KD Validated
human	WB	J Exp Clin Cancer Res; PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer; Authors - Liang Xu
mouse	WB	Nat Commun; CSN6 drives carcinogenesis by positively regulating Myc stability.; Authors - Jian Chen

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