VPRBP Polyclonal antibody

• Featured Product
• KD/KO Validated

VPRBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

42

Reviews (3)

Cat no : 11612-1-AP

Synonyms

KIAA0800, HIV-1 Vpr-binding protein, HIV 1 Vpr binding protein, EC:2.7.11.1, DDB1- and CUL4-associated factor 1

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Validation Data Gallery

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  WB analysis using 11612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11612-1-AP

  WB result of VPRBP antibody (11612-1-AP; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPRBP transfected HEK-293 cells.

• 

  WB analysis of HeLa using 11612-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11612-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11612-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11612-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11612-1-AP (VPRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11612-1-AP

  IP result of anti-VPRBP (IP:11612-1-AP, 3ug; Detection:11612-1-AP 1:1000) with HeLa cells lysate 3800ug.

• 

  IHC staining of mouse testis using 11612-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11612-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11612-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11612-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11612-1-AP (VPRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11612-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11612-1-AP (VPRBP antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 11612-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using VPRBP antibody (11612-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 11612-1-AP

  1X10^6 HeLa cells were stained with 0.2ug VPRBP antibody (11612-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: DU 145 cells, HeLa cells, mouse testis tissue, PC-3 cells, HEK-293 cells, K-562 cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue, mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 20 publications below
• WB: See 38 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• IP: See 4 publications below

Product Information

11612-1-AP targets VPRBP in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VPRBP fusion protein Ag2184
• Full Name: Vpr (HIV-1) binding protein
• Calculated Molecular Weight: 1506 aa, 169 kDa
• Observed Molecular Weight: 169 kDa
• GenBank Accession Number: BC022792
• Gene Symbol: VPRBP
• Gene ID (NCBI): 9730
• RRID: AB_2216933
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VprBP was first identified as a protein that can interact with HIV-1 viral protein R (PMID: 11223251). It is a component of the CUL4A-RBX1-DDB1-VprBP/DCAF1 E3 ubiquitin-protein ligase complex that could interact with HIV-1 virus Vpr protein and HIV-2 virus Vpx protein (PMID: 18332868; 17314515; 18606781). VprBP is a 1,507-amino acid protein that contains conserved domains, including YXXY repeats, the Lis homology motif, and WD40 repeats. Through binding to Vpr, VprBP allows Vpr to modulate the catalytic activity of the CUL4-DDB1 complex, which in turn leads to the induction of G2 phase arrest in the virus-infected cells (PMID: 17630831). Recently it has been reported that VprBP is able to regulate the p53-induced transcription and apoptotic pathway (PMID: 22184063).

Publications

Species	Application	Title
human	WB,IHC,IF	Science; CRL4 complex regulates mammalian oocyte survival and reprogramming by activation of TET proteins.; Authors - Chao Yu ; KD Validated
mouse, human	WB, IF	Mol Cell; CRL4(VprBP) E3 Ligase Promotes Monoubiquitylation and Chromatin Binding of TET Dioxygenases.; Authors - Tadashi Nakagawa ; KD Validated
human	WB	Mol Cell; Vpr Targets TET2 for Degradation by CRL4VprBP E3 Ligase to Sustain IL-6 Expression and Enhance HIV-1 Replication.; Authors - Lei Lv ; KD Validated
human	WB	Nat Microbiol; Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.; Authors - Leonid Yurkovetskiy
human	WB	Cell Host Microbe; Vpr overcomes macrophage-specific restriction of HIV-1 Env expression and virion production.; Authors - Michael Mashiba
human	WB, IF	Cell Host Microbe; Cyclin L2 Is a Critical HIV Dependency Factor in Macrophages that Controls SAMHD1 Abundance.; Authors - George Boateng Kyei ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Santosh (Verified Customer) (10-24-2024)

Antibody works well with quite nice in westernblot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000

FH

Roy (Verified Customer) (06-13-2024)

Great antibody detecting DCAF1 (VPRBP) validated in our coIP/WB experiments (1/1000 diluation - 1h incubation at RT)

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HeLa cells

FH

Nikhil (Verified Customer) (09-17-2019)

Very good for both IP and western blot.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Colon cancer cells

VPRBP Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in Colon cancer cells (Cat no:11612-1-AP)

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