CUL2 Polyclonal antibody

• Featured Product
• KD/KO Validated

CUL2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 10981-2-AP

Synonyms

CUL 2, CUL2, cullin 2

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Validation Data Gallery

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  WB analysis using 10981-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10981-2-AP (CUL2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10981-2-AP

  WB result of CUL2 antibody (10981-2-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CUL2 transfected PC-3 cells.

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  IP experiment of HeLa using 10981-2-AP

  IP result of anti-CUL2 (IP:10981-2-AP, 4ug; Detection:10981-2-AP 1:2000) with HeLa cells lysate 1360 ug.

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  IHC staining of human prostate cancer using 10981-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10981-2-AP (CUL2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10981-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10981-2-AP (CUL2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HEK-293 cells, PC-3 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 10 publications below
• CoIP: See 1 publications below

Product Information

10981-2-AP targets CUL2 in WB, IP, IHC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: CUL2 fusion protein Ag1434
• Full Name: cullin 2
• Calculated Molecular Weight: 87 kDa
• Observed Molecular Weight: 87 kDa
• GenBank Accession Number: BC009591
• Gene Symbol: CUL2
• Gene ID (NCBI): 8453
• RRID: AB_2086422
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The cullin family proteins are scaffold proteins for the Ring finger type E3 ligases, participating in the proteolysis through the ubiquitin-proteasome pathway. Humans express seven cullin proteins: CUL1-3, CUL4A, CUL4B, CUL5, and CUL7. Each cullin protein can form an E3 ligase similar to the prototype Ring-type E3 ligase Skp1-CUL1-F-box complex. The Cullin-RING-finger type E3 ligases are important regulators in early embryonic development, as highlighted by genetic studies demonstrating that knock-out of CUL1, CUL3, or CUL4A in mice results in early embryonic lethality.

Publications

Species	Application	Title
human	WB	Dev Cell; AMBRA1 interplay with cullin E3 ubiquitin ligases regulates autophagy dynamics.; Authors - Manuela Antonioli ; KD Validated
human	WB	Cell Death Differ; CRL2-KLHDC3 E3 ubiquitin ligase complex suppresses ferroptosis through promoting p14ARF degradation.; Authors - Pingzhao Zhang
human	WB	Oncogene; iASPP is essential for HIF-1α stabilization to promote angiogenesis and glycolysis via attenuating VHL-mediated protein degradation.; Authors - Dong Zhao
human	WB	Aging (Albany NY); Tumor suppressor DCAF15 inhibits epithelial-mesenchymal transition by targeting ZEB1 for proteasomal degradation in hepatocellular carcinoma.; Authors - Xiao Dong ; KD Validated
human	WB	Mol Cell Biol; DNA damage regulates UHRF1 stability via the SCF(β-TrCP) E3 ligase.; Authors - Chen Hao H ; KD Validated
human	WB	J Cell Sci; Cullin-3-KCTD10-mediated CEP97 degradation promotes primary cilium formation.; Authors - Tomoaki Nagai ; KD Validated

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