Anticorps Polyclonal de lapin anti-Collagen Type III (N-terminal)

• Phare
• Validé par KD/KO

Collagen Type III (N-terminal) Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (6)

Applications

WB, IHC, IF-P, IF-Fro, IP, ELISA

Conjugaison

Non conjugué

Publications

410

Avis (5)

N° de cat : 22734-1-AP

Synonymes

COL3A1, COL3, collagen, type III, alpha 1, Collagen Type III, Collagen alpha-1(III) chain

---

Galerie de données de validation

• 

  WB analysis of mouse skin using 22734-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat skin using 22734-1-AP

  rat skin tissue were subjected to SDS PAGE followed by western blot with 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skin using 22734-1-AP

  IP result of anti-Collagen Type III (N-terminal) (IP:22734-1-AP, 4ug; Detection:22734-1-AP 1:500) with mouse skin tissue lysate 3200ug.

• 

  IHC staining of human hepatocirrhosis using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human malignant melanoma using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human malignant melanoma using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human colon using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human skin using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 10x lens).

• 

  IHC staining of human skin using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody at dilution of 1:1000 (under 40x lens).

• 

  IHC staining of human skin cancer using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin cancer using 22734-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 22734-1-AP (Collagen Type III (N-terminal) antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon using 22734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Cytokeratin 20 antibody (60183-1-Ig, Clone: 4D10A4, Magenta), CoraLite®594 CD68 antibody (CL594-25747, red).

• 

  IF Staining of human colon using 22734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human colon using 22734-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human colon tissue using Collagen Type III (N-terminal) antibody (22734-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse colon using 22734-1-AP

  Immunofluorescence of mouse colon: Frozen OCT-embedded mouse colon sections were stained with anti-E-cadherin antibody (20874-1-AP) labeled with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2, green) in the frist step,anti-CD45 antibody (80297-1-RR) labeled with FlexAble CoraLite Plus 555 Kit (KFA002, orange) in the second step,anti-Collagen Type III antibody (22734-1-AP) labeled with FlexAble CoraLite647 Kit (KFA003, magenta) in the third step, and DAPI (blue).

---

Applications testées

• Résultats positifs en WB: tissu cutané de souris, tissu cutané de rat
• Résultats positifs en IP: tissu cutané de souris
• Résultats positifs en IHC: tissu de cirrhose hépatique humain, tissu cardiaque de souris, tissu cutané humain, tissu de cancer de la peau humain, tissu de cancer du pancréas humain, tissu de côlon de souris, tissu de côlon humain, tissu de mélanome malin humain, tissu hépatique de souris, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon humain
• Résultats positifs en IF-Fro: tissu de côlon de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 292 publications below
• IHC: See 122 publications below
• IF: See 66 publications below

Informations sur le produit

22734-1-AP cible Collagen Type III (N-terminal) dans les applications de WB, IHC, IF-P, IF-Fro, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, bovin, canin, Humain, Lapin, porc, poulet, souris, Hamster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Collagen Type III (N-terminal) Protéine recombinante Ag18658
• Nom complet: collagen, type III, alpha 1
• Masse moléculaire calculée: 1466 aa, 139 kDa
• Poids moléculaire observé: 140-180 kDa
• Numéro d’acquisition GenBank: BC028178
• Symbole du gène: COL3A1
• Identification du gène (NCBI): 1281
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Type III collagen is a fibrillar forming collagen comprising three α1(III) chains and is expressed in early embryos and throughout embryogenesis (PMID: 9050868). In the adult, type III collagen is a major component of the extracellular matrix in a variety of internal organs and skin. It occurs in most soft connective tissues along with type I collagen (PMID: 2445760). COL3A1 gene encodes type III procollagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms (PMID: 10706896; 2243125; 18389341). This antibody raised against 24-152 aa of prepro α1 (III) chain of human type III procollagen detects type III procollagen at 140-180 kDa and also in some lysates reveals a 70-kDa band which has been reported and may represent a cleaved form of type III procollagen (PMID: 17424834; 19648160; 22802960).

Publications

Species	Application	Title
rat	WB	Cell Metab; Sphingolipid metabolism controls mammalian heart regeneration; Authors - Xiaoqian Ji
mouse	IHC	Acta Pharm Sin B; SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.; Authors - Fen Long
	WB,IHC	Small; M2 Macrophage Membrane-Mediated Biomimetic-Nanoparticle Carrying COX-siRNA Targeted Delivery for Prevention of Tendon Adhesions by Inhibiting Inflammation; Authors - Jie Sun
mouse,human	WB	Basic Res Cardiol; SAIL: a new conserved anti-fibrotic lncRNA in the heart.; Authors - Shenjian Luo
mouse	WB	J Exp Med; EP3 enhances adhesion and cytotoxicity of NK cells toward hepatic stellate cells in a murine liver fibrosis model.; Authors - Xixi Tao
human	WB	Drug Des Devel Ther; Keloid Patient Plasma-Derived Exosomal hsa_circ_0020792 Promotes Normal Skin Fibroblasts Proliferation, Migration, and Fibrogenesis via Modulating miR-193a-5p and Activating TGF-β1/Smad2/3 Signaling; Authors - Huan Hu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Guennec (Verified Customer) (10-12-2023)

I have 2 bands at 70 KDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse Heart Tissue

Collagen Type III (N-terminal) Antibody Western Blot validation (1/1000 dilution) in Mouse Heart Tissue (Cat no:22734-1-AP)

FH

Sarah-Eve (Verified Customer) (02-19-2023)

Works well. One band observed at 180 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Human right ventricular fibroblasts

FH

Gayatri (Verified Customer) (06-10-2022)

Good signal but inexplicable molecular weight. Observe bands at 70 kDa instead of expected 140-180 kDa although this product is validated for Rat. Observed in both rat heart and tail lysates.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Rat Heart Tissue

FH

Yuki (Verified Customer) (03-30-2022)

Works well for IF application and presented with intense staining

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: skin cells

Collagen Type III (N-terminal) Antibody Immunohistochemistry validation (1:50 dilution) in skin cells (Cat no:22734-1-AP)

FH

Ryan (Verified Customer) (04-20-2018)

Antibody worked well for IF application and presented with intense staining of both medial and adventitial collagen in artery sections

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: paraffin carotid artery section

Collagen Type III (N-terminal) Antibody Immunofluorescence validation (1:400 dilution) in paraffin carotid artery section (Cat no:22734-1-AP)

Produits associés

Collagen Type III (C-terminal) Polyclonal antibody

13548-1-AP

Collagen Type I Polyclonal antibody

14695-1-AP

Collagen Type II Polyclonal antibody

15943-1-AP

Collagen Type IV Polyclonal antibody

10807-1-AP

MMP1 Polyclonal antibody

10371-2-AP

Fibronectin Polyclonal antibody

15613-1-AP

Fibronectin Monoclonal antibody

66042-1-Ig