Anticorps Polyclonal de lapin anti-Claudin 18

Claudin 18 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

7

N° de cat : 21126-1-AP

Synonymes

claudin 18, CLDN18, SFTA5, SFTPJ

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Galerie de données de validation

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  WB analysis of mouse kidney using 21126-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21126-1-AP (Claudin 18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse stomach using 21126-1-AP

  IP result of anti-Claudin 18 (IP:21126-1-AP, 3ug; Detection:21126-1-AP 1:500) with mouse stomach tissue lysate 4000ug.

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  IHC staining of human stomach using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21126-1-AP (Claudin 18 antibody) at dilution of 1:1000 (under 10x lens).

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  IHC staining of human stomach using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 21126-1-AP (Claudin 18 antibody) at dilution of 1:1000 (under 40x lens).

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  IHC staining of human stomach cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human ovary tumor using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21126-1-AP (Claudin 18 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 21126-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21126-1-AP (Claudin 18 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MKN-45 using 21126-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed MKN-45 cells using 21126-1-AP (Claudin 18 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu rénal de souris
• Résultats positifs en IP: tissu d'estomac de souris
• Résultats positifs en IHC: tissu d'estomac humain, tissu de cancer de l'estomac humain, tissu de cancer du pancréas humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MKN-45,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Informations sur le produit

21126-1-AP cible Claudin 18 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Claudin 18 Protéine recombinante Ag15517
• Nom complet: claudin 18
• Masse moléculaire calculée: 261 aa, 28 kDa
• Poids moléculaire observé: 28-29 kDa
• Numéro d’acquisition GenBank: BC146668
• Symbole du gène: CLDN18
• Identification du gène (NCBI): 51208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Claudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 11585919; 18036336). Claudins exhibit specific patterns of expression in different tissues (PMID: 15447685). CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific (PMID: 11585919; 21832145). Altered CLDN18 expression has been reported in various human malignancies, including gastric cancer, lung adenocarcinoma, and pancreatic neoplasm (PMID: 17459057; 22076167; 21832145). This antibody can recognize both CLDN18.1 and CLDN18.2.

Publications

Species	Application	Title
human	IHC	Cell Rep Med; Personalized drug screening in patient-derived organoids of biliary tract cancer and its clinical application; Authors - Xiaoxue Ren
mouse	IHC	Ann Transl Med; Inhibition of protein kinase C alpha attenuates lipopolysaccharide-triggered acute lung injury by alleviating the hyperinflammatory response and oxidative stress.; Authors - Yang Chen
mouse	WB,IF	Int J Mol Med; Investigating the regulatory role of ORMDL3 in airway barrier dysfunction using in vivo and in vitro models.; Authors - Ruixue Yang
mouse	IF	BMC Pulm Med; SPAK-p38 MAPK signal pathway modulates claudin-18 and barrier function of alveolar epithelium after hyperoxic exposure.; Authors - Chih-Hao Shen
rat	IF	J Stroke Cerebrovasc Dis; Effect of MicroRNA-126a-3p on Bone Marrow Mesenchymal Stem Cells Repairing Blood-brain Barrier and Nerve Injury after Intracerebral Hemorrhage.; Authors - Chunyan Wang
human	IHC	Cancers (Basel); Primary Colorectal Tumor Displays Differential Genomic Expression Profiles Associated with Hepatic and Peritoneal Metastases; Authors - Maximiliano Gelli

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