Validation Data Gallery
|Positive WB detected in||human testis tissue|
|Positive IHC detected in||human testis tissue, mouse brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12152-1-AP targets Claudin 11 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Claudin 11 fusion protein Ag2764|
|Full Name||claudin 11|
|Calculated molecular weight||207 aa, 22 kDa|
|Observed molecular weight||22 kDa|
|GenBank accession number||BC013577|
|Gene ID (NCBI)||5010|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.
|Product Specific Protocols|
|WB protocol for Claudin 11 antibody 12152-1-AP||Download protocol|
|IHC protocol for Claudin 11 antibody 12152-1-AP||Download protocol|
|IF protocol for Claudin 11 antibody 12152-1-AP||Download protocol|
|FC protocol for Claudin 11 antibody 12152-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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