Claudin 11 Polyclonal antibody

Claudin 11 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12152-1-AP

Synonyms

claudin 11, CLDN11, OSP, OTM

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Validation Data Gallery

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  WB analysis of human testis using 12152-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12152-1-AP (Claudin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 12152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12152-1-AP (Claudin 11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12152-1-AP (Claudin 11 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse brain using 12152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12152-1-AP (Claudin 11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12152-1-AP (Claudin 11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12152-1-AP

  Immunofluorescent analysis of HepG2 cells, using CLDN11 antibody 12152-1-AP at 1:100 dilution and PE-labeled goat anti-rabbit IgG(red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: human testis tissue
• Positive IHC detected in: human testis tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

12152-1-AP targets Claudin 11 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Claudin 11 fusion protein Ag2764
• Full Name: claudin 11
• Calculated Molecular Weight: 207 aa, 22 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC013577
• Gene Symbol: Claudin 11
• Gene ID (NCBI): 5010
• RRID: AB_10694425
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Publications

Species	Application	Title
human	IF	Mol Cell Endocrinol; Cardiotonic steroid ouabain stimulates expression of blood-testis barrier proteins claudin-1 and -11 and formation of tight junctions in Sertoli cells.; Authors - Raimund Dietze
rat	WB	Environ Sci Pollut Res Int; Polystyrene microplastics induce blood-testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats.; Authors - Shengda Li
human	WB	J Physiol Biochem; α2,6-Sialylation promotes hepatocellular carcinoma cells migration and invasion via enhancement of nSmase2-mediated exosomal miRNA sorting; Authors - Liping Wang
human	WB	Sci Rep; Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis.; Authors - Zhaoxing Li

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