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Claudin 23 Polyclonal antibody

Claudin 23 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25296-1-AP

Synonyms

claudin 23, CLDN23, CLDNL, hCG1646163

Validation Data Gallery

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  • Western Blot (WB) analysis of COLO 320 cells using Claudin 23 Polyclonal antibody (25296-1-AP)

    WB analysis of COLO 320 using 25296-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25296-1-AP (Claudin 23 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Claudin 23 Polyclonal antibody (25296-1-AP)

    IHC staining of human colon cancer using 25296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25296-1-AP (Claudin 23 antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using Claudin 23 Polyclonal antibody (25296-1-AP)

    IHC staining of human colon cancer using 25296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25296-1-AP (Claudin 23 antibody at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25296-1-AP is Claudin 23 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, canine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 23 fusion protein Ag18189
Full Name claudin 23
Calculated Molecular Weight 292 aa, 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC125148
Gene Symbol Claudin 23
Gene ID (NCBI) 137075
RRIDAB_2880013
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudin 23 (CLDN23) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 12736707; 18036336). Claudins exhibit specific patterns of expression in different tissues. Human CLDN23 mRNA was expressed in germinal center B cells, placenta, stomach as well as in colon tumor (PMID: 12736707). CLDN23 has been reported as a candidate tumor suppressor gene implicated in intestinal-type gastric cancer (PMID: 12736707).

Protocols

Product Specific Protocols
WB protocol for Claudin 23 antibody 25296-1-APDownload protocol
IHC protocol for Claudin 23 antibody 25296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,canineWB,IF

Life Sci

Microbial metabolite n-butyrate upregulates intestinal claudin-23 expression through SP1 and AMPK pathways in mouse colon and human intestinal Caco-2 cells

Authors - Wenxi Xu
View Article