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  • KD/KO Validated

Claudin 1 Polyclonal antibody

Claudin 1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat, canine and More (5)





Cat no : 13050-1-AP


claudin 1, CLD1, CLDN1, ILVASC, SEMP1

Tested Applications

Positive WB detected inA431 cells, mouse skin tissue, mouse thymus tissue, mouse liver tissue, rat skin tissue, A549 cells, HUVEC cells
Positive IHC detected inhuman cervical cancer tissue, human skin cancer tissue, mouse skin tissue, human oesophagus cancer tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHUVEC cells, MDCK cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13050-1-AP targets Claudin 1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat, goat, chicken, sheep, pig, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 1 fusion protein Ag3713
Full Name claudin 1
Calculated Molecular Weight 211 aa, 23 kDa
Observed Molecular Weight 20-23 kDa
GenBank Accession NumberBC012471
Gene Symbol CLDN1
Gene ID (NCBI) 9076
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is expressed primarily in keratinocytes and normal mammary epithelial cells.


Product Specific Protocols
WB protocol for Claudin 1 antibody 13050-1-APDownload protocol
IHC protocol for Claudin 1 antibody 13050-1-APDownload protocol
IF protocol for Claudin 1 antibody 13050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Cell Host Microbe

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

Authors - Zhenyu Tian

Clin Cancer Res

ZIP4 Promotes Pancreatic Cancer Progression by Repressing ZO-1 and claudin-1 through a ZEB1-Dependent Transcriptional Mechanism.

Authors - Mingyang Liu


O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.

Authors - Ieva Bagdonaite

Mater Today Bio

Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis

Authors - Jingzhe Wang

PLoS Pathog

Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2.

Authors - Suzuki Ryosuke R


Baizhu shaoyao decoction restores the intestinal barrier and brain-gut axis balance to alleviate diarrhea-predominant irritable bowel syndrome via FoxO1/FoxO3a

Authors - Yuanyuan Wei


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


James (Verified Customer) (01-06-2022)

Works good. Nice image

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Gut
Claudin 1 Antibody Immunohistochemistry validation (1:100 dilution) in Gut (Cat no:13050-1-AP)

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with background. Staining is a mix of junctional (bright) and cytosolic (dim).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Keratinocytes
Claudin 1 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:13050-1-AP)