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Claudin 4-specific Polyclonal antibody

Claudin 4-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, RIP, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 16195-1-AP

Synonyms

claudin 4, Claudin 4-specific, CLDN4, CPE R, CPE receptor, CPER, CPETR, CPETR1, hCPE R, WBSCR8



Tested Applications

Positive WB detected inMDA-MB-453s cells, mouse small intestine tissue, human placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16195-1-AP targets Claudin 4-specific in WB, RIP, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name claudin 4
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberNM_001305
Gene Symbol CLDN4
Gene ID (NCBI) 1364
RRIDAB_2082969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They are small (20-27 kDa) proteins with very similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 4.

Protocols

Product Specific Protocols
WB protocol for Claudin 4-specific antibody 16195-1-APDownload protocol
IHC protocol for Claudin 4-specific antibody 16195-1-APDownload protocol
IF protocol for Claudin 4-specific antibody 16195-1-APDownload protocol
IP protocol for Claudin 4-specific antibody 16195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

Cell Metab

Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes

Authors - Junpeng Yang
mouse,ratWB,IHC

J Hazard Mater

Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.

Authors - Junrong Wu
humanWB

Free Radic Biol Med

Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells.

Authors - Restituto Tocmo
humanWB, RIP

Cancers (Basel)

Targeting PVT1 Exon 9 Re-Expresses Claudin 4 Protein and Inhibits Migration by Claudin-Low Triple Negative Breast Cancer Cells.

Authors - Fayola Levine
ratWB

EBioMedicine

Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis.

Authors - Yingchun Luo
humanCoIP

Lab Invest

NSUN2-mediated m5C methylation impairs endometrial receptivity

Authors - Jiafeng Lu