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Claudin 9-specific Polyclonal antibody

Claudin 9-specific Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16196-1-AP

Synonyms

claudin 9, CLDN9

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using Claudin 9-specific Polyclonal antibody (16196-1-AP)

    WB analysis of HeLa using 16196-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16196-1-AP (Claudin 9-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using Claudin 9-specific Polyclonal antibody (16196-1-AP)

    IP experiment of HeLa using 16196-1-AP

    IP result of anti-Claudin 9-specific (IP:16196-1-AP, 4ug; Detection:16196-1-AP 1:300) with HeLa cells lysate 880ug.

Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16196-1-AP targets Claudin 9-specific in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name claudin 9
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 25-29 kDa
GenBank Accession NumberNM_020982
Gene Symbol Claudin 9
Gene ID (NCBI) 9080
RRIDAB_2083123
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 9.

Protocols

Product Specific Protocols
WB protocol for Claudin 9-specific antibody 16196-1-APDownload protocol
IP protocol for Claudin 9-specific antibody 16196-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB, IHC

Histochem Cell Biol

Complexity and developmental changes in the expression pattern of claudins at the blood-CSF barrier.

Authors - Kratzer Ingrid I
View Article
humanWB

Front Genet

Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma.

Authors - Zezhong Mou
View Article