Anticorps Polyclonal de lapin anti-CENPJ

• Phare
• Validé par KD/KO

CENPJ Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

49

N° de cat : 11517-1-AP

Synonymes

BM032, CENP J, CENPJ, centromere protein J, CPAP, LAP, LIP1, LYST interacting protein 1, MCPH6

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Galerie de données de validation

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  WB analysis of mouse testis using 11517-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11517-1-AP (CENPJ Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11517-1-AP

  WB result of CENPJ antibody (11517-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CENPJ transfected Jurkat cells.

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  IF Staining of HepG2 using 11517-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CENPJ antibody (11517-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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  IF Staining of HepG2 using 11517-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 11517-1-AP (CENPJ antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Red: CL555-phalloidin staining of F-actin. .

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 19 publications below
• IHC: See 1 publications below
• IF: See 41 publications below

Informations sur le produit

11517-1-AP cible CENPJ dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CENPJ Protéine recombinante Ag2078
• Nom complet: centromere protein J
• Masse moléculaire calculée: 1338 aa, 153 kDa
• Poids moléculaire observé: 153 kDa
• Numéro d’acquisition GenBank: BC024209
• Symbole du gène: CENPJ
• Identification du gène (NCBI): 55835
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CENPJ, also named as CPAP, LAP and LIP1, belongs to the TCP10 family. It plays an important role in cell division and centrosome function by participating in centriole duplication. CENPJ inhibits microtubule nucleation from the centrosome.

Publications

Species	Application	Title
human	IF	Nature; TRIM37 controls cancer-specific vulnerability to PLK4 inhibition.; Authors - Franz Meitinger
human	IF	Science; Cell biology. Reversible centriole depletion with an inhibitor of Polo-like kinase 4.; Authors - Yao Liang Wong
mouse	WB,IF	Nat Cell Biol; The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.; Authors - Huijie Zhao
mouse	WB,IF	Neuron; Microcephaly Proteins Wdr62 and Aspm Define a Mother Centriole Complex Regulating Centriole Biogenesis, Apical Complex, and Cell Fate.; Authors - Divya Jayaraman
rat	IF	Dev Cell; SAS-6 Assembly Templated by the Lumen of Cartwheel-less Centrioles Precedes Centriole Duplication.; Authors - Chii Shyang Fong
human	IF	Nat Commun; Selective autophagy maintains centrosome integrity and accurate mitosis by turnover of centriolar satellites.; Authors - Søs Grønbæk Holdgaard

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