SASS6 Polyclonal antibody

SASS6 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 21377-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, IP, ELISA

HsSAS 6, HsSAS-6, SAS 6, SAS6, Spindle assembly abnormal protein 6 homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, human testis tissue, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21377-1-AP targets SASS6 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SASS6 fusion protein Ag15917 Predict reactive species
Full Name spindle assembly 6 homolog (C. elegans)
Calculated Molecular Weight 657 aa, 74 kDa
Observed Molecular Weight 70-74 kDa
GenBank Accession NumberBC101026
Gene Symbol SASS6
Gene ID (NCBI) 163786
RRIDAB_2878851
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ6UVJ0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Spindle assembly abnormal protein 6 homolog (SASS6) is a centrosomal protein that, together with PLK4, CEP135, CENPJ (also known as CPAP) and STIL, is involved in regulating the number of centrosomes in human cells.

Protocols

Product Specific Protocols
WB protocol for SASS6 antibody 21377-1-APDownload protocol
IHC protocol for SASS6 antibody 21377-1-APDownload protocol
IP protocol for SASS6 antibody 21377-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Curr Biol

SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.

Authors - Hindol Gupta
humanWB,IHC

Oncol Rep

Knockdown of SASS6 reduces growth of MDA‑MB‑231 triple‑negative breast cancer cells through arrest of the cell cycle at the G2/M phase.

Authors - Lili Du
humanIF

Science

The mechanism of acentrosomal spindle assembly in human oocytes

Authors - Tianyu Wu
humanIF

EBioMedicine

Bi-allelic variants in CEP295 cause Seckel-like syndrome presenting with primary microcephaly, developmental delay, intellectual disability, short stature, craniofacial and digital abnormalities

Authors - Niu Li
humanWB

Clin Exp Med

SASS6 promotes tumor proliferation and is associated with TP53 and immune infiltration in lung adenocarcinoma

Authors - Zihao Li
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