SASS6 Polyclonal antibody

SASS6 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 21377-1-AP

Synonyms

HsSAS 6, SAS 6, SAS6, SASS6

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Validation Data Gallery

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  WB analysis of HeLa using 21377-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21377-1-AP (SASS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 21377-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21377-1-AP (SASS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21377-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21377-1-AP (SASS6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 21377-1-AP

  IP result of anti-SASS6 (IP:21377-1-AP, 4ug; Detection:21377-1-AP 1:1000) with HeLa cells lysate 600ug.

• 

  IP experiment of HeLa cells using 21377-1-AP

  IP result of anti-SAS6 (21377-1-AP for IP and Detection) with HeLa cells.

• 

  IHC staining of mouse testis using 21377-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21377-1-AP (SASS6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 21377-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21377-1-AP (SASS6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21377-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 21377-1-AP (SASS6 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: HeLa cells, human testis tissue, MCF-7 cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse testis tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

21377-1-AP targets SASS6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SASS6 fusion protein Ag15917
• Full Name: spindle assembly 6 homolog (C. elegans)
• Calculated Molecular Weight: 657 aa, 74 kDa
• Observed Molecular Weight: 70-74 kDa
• GenBank Accession Number: BC101026
• Gene Symbol: SASS6
• Gene ID (NCBI): 163786
• RRID: AB_2878851
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Curr Biol; SAS-6 Association with γ-Tubulin Ring Complex Is Required for Centriole Duplication in Human Cells.; Authors - Hindol Gupta
human	WB, IHC	Oncol Rep; Knockdown of SASS6 reduces growth of MDA‑MB‑231 triple‑negative breast cancer cells through arrest of the cell cycle at the G2/M phase.; Authors - Lili Du
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
human	IF	EBioMedicine; Bi-allelic variants in CEP295 cause Seckel-like syndrome presenting with primary microcephaly, developmental delay, intellectual disability, short stature, craniofacial and digital abnormalities; Authors - Niu Li