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CP110 Polyclonal antibody
CP110 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 12780-1-AP
Validation Data Gallery
|Positive WB detected in||HCT 116 cells, HeLa cells, HEK-293T cells, Jurkat cells, MOLT-4 cells|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:12000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:3000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:250-1:1000|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
12780-1-AP targets CP110 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.
|Tested Reactivity||human, mouse, rat, Canine|
|Cited Reactivity||human, mouse, rat, canine, Drosophila, Xenopus, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||CP110 fusion protein Ag3489|
|Full Name||CP110 protein|
|Calculated molecular weight||991 aa, 111 kDa|
|Observed molecular weight||113 kDa|
|GenBank accession number||BC036654|
|Gene ID (NCBI)||9738|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
CP110, also named as CCP110 and KIAA0419, is an 110kDa protein. This gene is different to the gene CEP110 (geneID:11064; CNTRL). CP110 is a centriolar protein that positively regulates centrioleduplication while restricting centriole elongation and ciliogenesis. And it acts as a key negative regulator of ciliogenesis in collaboration with CEP97 by capping the mother centriole thereby preventing cilia formation.
|Product Specific Protocols|
|WB protocol for CP110 antibody 12780-1-AP||Download protocol|
|IHC protocol for CP110 antibody 12780-1-AP||Download protocol|
|IF protocol for CP110 antibody 12780-1-AP||Download protocol|
|IP protocol for CP110 antibody 12780-1-AP||Download protocol|
|Click here to view our Standard Protocols|
A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.
USP33 regulates centrosome biogenesis via deubiquitination of the centriolar protein CP110.
Intrinsic bioactivity of black phosphorus nanomaterials on mitotic centrosome destabilization through suppression of PLK1 kinase.
Nat Cell Biol
miR-129-3p controls cilia assembly by regulating CP110 and actin dynamics.
Nat Cell Biol
CEP162 is an axoneme-recognition protein promoting ciliary transition zone assembly at the cilia base.
Nat Cell Biol
The Cep63 paralogue Deup1 enables massive de novo centriole biogenesis for vertebrate multiciliogenesis.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Elisa (Verified Customer) (01-05-2023)
RPE1 cells stained for Hoechst (DNA marker, in green), CP110 (in magenta) and gtub (pericentriolar matrix marker, in green). RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).
Sarah (Verified Customer) (02-16-2021)
Works very well for detection of CP110 protein using western blotting and immunfluorescence
EK (Verified Customer) (05-04-2020)
works well for immunofluorescence detection of basal body localization