PLK2 Polyclonal antibody

• Featured Product
• KD/KO Validated

PLK2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15956-1-AP

Synonyms

PLK 2, PLK2, Polo like kinase 2, Serum inducible kinase, SNK

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Validation Data Gallery

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  WB analysis using 15956-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15956-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15956-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15956-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 15956-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 15956-1-AP (PLK2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 15956-1-AP

  IP result of anti-PLK2 (IP:15956-1-AP, 4ug; Detection:15956-1-AP 1:300) with HeLa cells lysate 2480ug.

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Tested Applications

• Positive WB detected in: HeLa cells, MCF-7 cells, COLO 320 cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IP: See 1 publications below

Product Information

15956-1-AP targets PLK2 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PLK2 fusion protein Ag8710
• Full Name: polo-like kinase 2 (Drosophila)
• Calculated Molecular Weight: 685 aa, 78 kDa
• Observed Molecular Weight: 75-80 kDa
• GenBank Accession Number: BC013879
• Gene Symbol: PLK2
• Gene ID (NCBI): 10769
• RRID: AB_2237367
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PLK2(Polo-like kinase 2) is also named as SNK and belongs to the Ser/Thr protein kinase family. It plays a role in normal cell division. Plk2 expression is detected only in G1, and endogenous Plk2 is rapidly turned over. Therefore, the expression of the three mammalian Plks during the cell cycle resembles the successive transition of the cyclins, prompting speculation that Plks may reg-ulate the cell cycle in a manner analogous to that of Cdks(PMID:12972611).

Publications

Species	Application	Title
human	WB	Oncotarget; MicroRNA-27b up-regulated by human papillomavirus 16 E7 promotes proliferation and suppresses apoptosis by targeting polo-like kinase2 in cervical cancer.; Authors - Fei Liu
rat	WB	J Biochem Mol Toxicol; PLK2 protects retinal ganglion cells from oxidative stress by potentiating Nrf2 signaling via GSK-3β.; Authors - Yazhi Fan ; KD Validated
	IP	Cell Biol Int; Tazarotene-induced gene 1 interacts with Polo-like kinase 2 and inhibits cell proliferation in HCT116 colorectal cancer cells.; Authors - Chun-Hua Wang