CEP350-Specific Polyclonal antibody

CEP350-Specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 20022-1-AP

Synonyms

Cep350, GM133, Centrosome-associated protein of 350 kDa, Centrosome-associated protein 350, centrosomal protein 350kDa

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Validation Data Gallery

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  WB analysis using 20022-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20022-1-AP (CEP350-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse liver using 20022-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 20022-1-AP (CEP350-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 20022-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CEP350-Specific antibody (20022-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Tested Applications

• Positive WB detected in: mouse heart tissue, mouse skeletal muscle tissue
• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20022-1-AP targets CEP350-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: centrosomal protein 350kDa
• Calculated Molecular Weight: 351 kDa
• Observed Molecular Weight: 245 kDa
• GenBank Accession Number: NM_014810
• Gene Symbol: CEP350
• Gene ID (NCBI): 9857
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP350, or Centrosomal Protein 350 kDa, also named CAP350, FLJ38282, FLJ44058, GM133, and KIAA0480, is a protein that plays a crucial role in the formation and maintenance of the centrosome, which is essential for cell division. The molecular weight of CEP350 varies depending on the isoforms and post-translational modifications. the molecular weight of CEP350 can range from 200-245 kDa, 143 kDa, to 65 kDa. These variations in molecular weight are likely due to different isoforms of the protein that arise from alternative splicing or other post-translational modifications.