CEP192 Polyclonal antibody

CEP192 Polyclonal Antibody for IF, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

12

Cat no : 18832-1-AP

Synonyms

centrosomal protein 192kDa, CEP192, KIAA1569, KIAA1569 protein

---

Validation Data Gallery

• 

  IP experiment of HeLa cells using 18832-1-AP

  IP result of anti-CEP192 (18832-1-AP for IP and Detection) with HeLa cells.

• 

  IHC staining of human liver cancer using 18832-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18832-1-AP (CEP192 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 18832-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18832-1-AP (CEP192 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of hTERT-RPE1 using 18832-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using CEP192 antibody (18832-1-AP) at dilution of 1:150 and Cy3-conjugated Affinipure Goat Anti-Rabbit IgG(H+L), SCLT1 antibody (14875-1-AP, Magenta), CoraLite®488 ARL13B antibody (CL488-17711, green).

• 

  IF Staining of HeLa using 18832-1-AP

  Immunofluorescent analysis of HeLa cells using 18832-1-AP (CEP192 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18832-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CEP192 antibody (18832-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18832-1-AP

  Immunofluorescent analysis of HeLa cells using 18832-1-AP (CEP192 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive IP detected in: HeLa cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: hTERT-RPE1 cells, HeLa cells

Recommended dilution

• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 12 publications below

Product Information

18832-1-AP targets CEP192 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: centrosomal protein 192kDa
• Calculated Molecular Weight: 213 kDa
• Observed Molecular Weight: 250-300 kDa
• GenBank Accession Number: BC144481
• Gene Symbol: CEP192
• Gene ID (NCBI): 55125
• RRID: AB_2878560
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP192, also named as PP8407 and KIAA1569, interacts with SHBG. It is required for mitotic centrosome and spindle assembly. CEP192 appears to be a major regulator of pericentriolar material (PCM) recruitment, centrosome maturation, and centriole duplication.

Publications

Species	Application	Title
mouse	IF	Science; Mechanism of spindle pole organization and instability in human oocytes.; Authors - Chun So
mouse	IF	Science; A liquid-like spindle domain promotes acentrosomal spindle assembly in mammalian oocytes.; Authors - Chun So
mouse	IF	EMBO Rep; ENKD1 promotes CP110 removal through competing with CEP97 to initiate ciliogenesis.; Authors - Ting Song
human	WB,IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
mouse	IF	PLoS Genet; Aurora kinase A is essential for meiosis in mouse oocytes.; Authors - Cecilia S Blengini
human	IF	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao