- Phare
- Validé par KD/KO
Anticorps Polyclonal de lapin anti-CD36
CD36 Polyclonal Antibody for WB, IHC, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Humain et plus (3)
Applications
WB, FC, IHC, ELISA
Conjugaison
Non conjugué
169
N° de cat : 18836-1-AP
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | plaquettes de sang périphérique humain, |
| Résultats positifs en IHC | tissu splénique humain, tissu cardiaque humain, tissu hépatique humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochimie (IHC) | IHC : 1:400-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| KD/KO | See 5 publications below |
| WB | See 145 publications below |
| IHC | See 24 publications below |
| FC | See 1 publications below |
Informations sur le produit
18836-1-AP cible CD36 dans les applications de WB, FC, IHC, ELISA et montre une réactivité avec des échantillons Humain
| Réactivité | Humain |
| Réactivité citée | bovin, Chèvre, Humain, porc |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | Peptide |
| Nom complet | CD36 molecule (thrombospondin receptor) |
| Masse moléculaire calculée | 472 aa, 53 kDa |
| Poids moléculaire observé | 88 kDa |
| Numéro d’acquisition GenBank | BC008406 |
| Symbole du gène | CD36 |
| Identification du gène (NCBI) | 948 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
Function
CD36, also known as platelet glycoprotein 4, is an integral membrane glycoprotein that acts as a scavenger receptor. CD36 can bind to multiple ligands, including thrombospondin-1, collagen, oxidized phospholipids, oxidized low-density lipoprotein, and long-chain fatty acids. CD36 can also bind to erythrocytes parasitized by Plasmodium falciparum and apoptotic cells. CD36 mediates different biological processes, acting as a signaling hub in angiogenesis, inflammatory response, and fatty acid metabolism.
Tissue specificity
CD36 is present on the surface of various cells types, such as adipocytes, monocytes, macrophages, platelets, microvascular endothelial cells, dendritic cells, and hematopoietic precursors of red cells.
Involvement in disease
Mutations in CD36 can give rise to platelet glycoprotein IV deficiency, a type of macrothrombocytopenia.
Polymorphisms in CD36 can increase susceptibility to malaria.
AAGIC haplotype at the CD36 locus increases the risk of coronary heart disease.
Disruption of CD36-dependent pathways and certain SNPs in the CD36 gene are attributed to impaired fatty acid metabolism, glucose intolerance, Alzheimer's disease, atherosclerosis, arterial hypertension, diabetes, and cardiomyopathy.
Isoforms
Apart from the full-length protein (isoform 1), one additional shorter isoform has been reported (PMID: 7509795). Other alternative isoforms have also been detected on the mRNA level (PMID: 17673938).
Post-translational modifications
The extracellular domain of CD36 is extensively glycosylated. Glycosylation is needed for the transport of CD36 to the plasma membrane, as well as mediating recognition and binding to ligands. Cytoplasmic tails of transmembrane domains can be phosphorylated and play a role in signal transduction. Intracellular domains can be additionally acetylated, ubiquitinated, and palmitoylated (PMID: 28919632).
Cellular localization
CD36 is present on the cell surface.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for CD36 antibody 18836-1-AP | Download protocol |
| IHC protocol for CD36 antibody 18836-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Suppression of angiopoietin-like 4 reprograms endothelial cell metabolism and inhibits angiogenesis | ||
Cell Rep Med Targeting neoadjuvant chemotherapy-induced metabolic reprogramming in pancreatic cancer promotes anti-tumor immunity and chemo-response | ||
Nat Commun The methyltransferase METTL3 negatively regulates nonalcoholic steatohepatitis (NASH) progression. | ||
Nat Commun Enhancement of anaerobic glycolysis - a role of PGC-1α4 in resistance exercise. | ||
Nat Commun Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH) |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Marsilius (Verified Customer) (02-24-2021) | Very clear signals, no nonspecific staining
 |
FH Maurice (Verified Customer) (10-24-2019) | Very clear signals, no undetermined bands like others antibody
|
