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  • KD/KO Validated

OLR1/LOX1 Polyclonal antibody

OLR1/LOX1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 11837-1-AP

Synonyms

OLR1, Lectin-like oxidized LDL receptor 1, hLOX-1, hLOX1, hLOX 1



Tested Applications

Positive WB detected inbEnd.3 cells, human liver tissue
Positive IP detected inL02 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11837-1-AP targets OLR1/LOX1 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OLR1/LOX1 fusion protein Ag2437
Full Name oxidized low density lipoprotein (lectin-like) receptor 1
Calculated Molecular Weight 273 aa, 31 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC022295
Gene Symbol OLR1
Gene ID (NCBI) 4973
RRIDAB_2299037
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OLR1/LOX-1 acts as a receptor, in the form of homodimer, that mediates the recognition, internalization, and degradation of oxidatively modified low density lipoprotein (oxLDL) (PMID: 9052782, 15695803). ORL1 is predominantly expressed in endothelial cells and vascular-rich organs such as the placenta, lung, liver, and brain (PMID: 9828121). OLR1 is a protein containing 273 amino acids with a calculated molecular mass of 31 kDa but an apparent molecular mass of 50 kDa, which may result from the glycosylation of four potential N-linked glycosylation sites located at the C-terminal domain (PMID: 9052782).

Protocols

Product Specific Protocols
WB protocol for OLR1/LOX1 antibody 11837-1-APDownload protocol
IHC protocol for OLR1/LOX1 antibody 11837-1-APDownload protocol
IP protocol for OLR1/LOX1 antibody 11837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Free Radic Biol Med

Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages

Authors - Wenchao Li,
humanWB

Cancer Lett

Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.

Authors - Bei Wang
  • KD Validated
humanWB

Metabolism

Deacetylation of Caveolin-1 by Sirt6 induces autophagy and retards high glucose-stimulated LDL transcytosis and atherosclerosis formation.

Authors - Ying Zhao
humanWB

Sci Rep

Lectin-like oxidized low-density lipoprotein receptor-1 facilitates metastasis of gastric cancer through driving epithelial-mesenchymal transition and PI3K/Akt/GSK3β activation.

Authors - Can Li
  • KD Validated
mouseIHC

Int J Cancer

The oxidized-LDL/LOX-1 axis in tumor endothelial cells enhances metastasis by recruiting neutrophils and cancer cells.

Authors - Takuya Tsumita
humanWB

Mol Cancer Res

OLR1 Promotes Pancreatic Cancer Metastasis via Increased c-Myc Expression and Transcription of HMGA2.

Authors - Gang Yang
  • KD Validated