OLR1 Polyclonal antibody

• Featured Product
• KD/KO Validated

OLR1 Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

38

Cat no : 11837-1-AP

Synonyms

CLEC8A, hLOX 1, Lectin like oxLDL receptor 1, LOX 1, LOX1, OLR1, Ox LDL receptor 1, SCARE1

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Validation Data Gallery

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  WB analysis using 11837-1-AP

  bEnd.3 cells were subjected to SDS PAGE followed by western blot with 11837-1-AP (OLR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11837-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11837-1-AP (OLR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of L02 using 11837-1-AP

  IP result of anti-OLR1 (IP:11837-1-AP, 4ug; Detection:11837-1-AP 1:800) with L02 cells lysate 1800ug.

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  IHC staining of human heart using 11837-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11837-1-AP (OLR1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 11837-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 11837-1-AP (OLR1 Antibody) at dilution of 1:200 (under 40x lens).

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  FC experiment of HUVEC using 11837-1-AP

  1X10^6 HUVEC cells were stained with 0.2ug OLR1 antibody (11837-1-AP, red) and control antibody (blue). Fixed with 4% PFA blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Tested Applications

• Positive WB detected in: bEnd.3 cells, human liver tissue
• Positive IP detected in: L02 cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HUVEC cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 10 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Product Information

11837-1-AP targets OLR1 in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: OLR1 fusion protein Ag2437
• Full Name: oxidized low density lipoprotein (lectin-like) receptor 1
• Calculated Molecular Weight: 273 aa, 31 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC022295
• Gene Symbol: OLR1
• Gene ID (NCBI): 4973
• RRID: AB_2299037
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OLR1/LOX-1 acts as a receptor, in the form of homodimer, that mediates the recognition, internalization, and degradation of oxidatively modified low density lipoprotein (oxLDL) (PMID: 9052782, 15695803). ORL1 is predominantly expressed in endothelial cells and vascular-rich organs such as the placenta, lung, liver, and brain (PMID: 9828121). OLR1 is a protein containing 273 amino acids with a calculated molecular mass of 31 kDa but an apparent molecular mass of 50 kDa, which may result from the glycosylation of four potential N-linked glycosylation sites located at the C-terminal domain (PMID: 9052782).

Publications

Species	Application	Title
mouse	WB	Free Radic Biol Med; Up-regulation of thioredoxin system by puerarin inhibits lipid uptake in macrophages; Authors - Wenchao Li,
human	WB	Cancer Lett; Up-regulation of OLR1 expression by TBC1D3 through activation of TNFα/NF-κB pathway promotes the migration of human breast cancer cells.; Authors - Bei Wang ; KD Validated
human	WB	Metabolism; Deacetylation of Caveolin-1 by Sirt6 induces autophagy and retards high glucose-stimulated LDL transcytosis and atherosclerosis formation.; Authors - Ying Zhao
human	WB	Sci Rep; Lectin-like oxidized low-density lipoprotein receptor-1 facilitates metastasis of gastric cancer through driving epithelial-mesenchymal transition and PI3K/Akt/GSK3β activation.; Authors - Can Li ; KD Validated
mouse	IHC	Int J Cancer; The oxidized-LDL/LOX-1 axis in tumor endothelial cells enhances metastasis by recruiting neutrophils and cancer cells.; Authors - Takuya Tsumita
human	WB	Mol Cancer Res; OLR1 Promotes Pancreatic Cancer Metastasis via Increased c-Myc Expression and Transcription of HMGA2.; Authors - Gang Yang ; KD Validated

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