SR-BI Polyclonal antibody

SR-BI Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 21277-1-AP

Synonyms

CD36 and LIMPII analogous 1, CD36 antigen like 1, CD36L1, CLA 1, CLA1, SCARB1, SRB1, SR-B1, SR-BI

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Validation Data Gallery

• 

  WB analysis of human brain using 21277-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21277-1-AP (SR-BI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 21277-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 21277-1-AP (SR-BI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 21277-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21277-1-AP (SR-BI antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 21277-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 21277-1-AP (SR-BI antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 21277-1-AP

  Immunofluorescent analysis of HepG2 cells, using SCARB1 antibody 21277-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human brain tissue, human testis tissue, HepG2 cells, mouse liver tissue
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 6 publications below

Product Information

The immunogen of 21277-1-AP is SR-BI Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SR-BI fusion protein Ag15759
• Full Name: scavenger receptor class B, member 1
• Calculated molecular weight: 552 aa, 61 kDa
• Observed molecular weight: 61-82 kDa
• GenBank accession number: BC022087
• Gene symbol: SCARB1
• Gene ID (NCBI): 949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
mouse	WB	Exp Ther Med; Effects of sepsis on the metabolism of sphingomyelin and cholesterol in mice with liver dysfunction.; Authors - Jiaqi Li
human	WB	J Physiol Biochem; ApoA-I induces S1P release from endothelial cells through ABCA1 and SR-BI in a positive feedback manner.; Authors - Xing Liu
mouse	WB	Autoimmunity; Inclisiran inhibits oxidized low-density lipoprotein-induced foam cell formation in Raw264.7 macrophages via activating the PPARγ pathway.; Authors - Zhaoping Wang
mouse	WB	Biochem Biophys Res Commun; TSH increases synthesis of hepatic ATP-binding cassette subfamily A member 1 in hypercholesterolemia.; Authors - Tiantian Zhang
mouse	WB	J Cell Mol Med; Photobiomodulation therapy promotes the ATP-binding cassette transporter A1-dependent cholesterol efflux in macrophage to ameliorate atherosclerosis.; Authors - Qianxia Yin
		J Virol; Neglected but Important Role of Apolipoprotein E Exchange in Hepatitis C Virus Infection.; Authors - Zaili Yang

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