Thrombospondin 1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Thrombospondin 1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

17

Reviews (1)

Cat no : 18304-1-AP

Synonyms

THBS, THBS 1, THBS1, thrombospondin 1, Thrombospondin-1, TSP, TSP 1, TSP1, TSP-1

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Validation Data Gallery

• 

  WB analysis using 18304-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human peripheral blood platelets using 18304-1-AP

  Human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 18304-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18304-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lung cancer using 18304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18304-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 18304-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 18304-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18304-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 18304-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18304-1-AP (Thrombospondin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: C6 cells, human peripheral blood platelets, HeLa cells, A431 cells, NIH/3T3 cells
• Positive IHC detected in: human lung cancer tissue, human placenta tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

18304-1-AP targets Thrombospondin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: thrombospondin 1
• Calculated molecular weight: 129 kDa
• Observed molecular weight: 180 kDa
• GenBank accession number: BC028145
• Gene symbol: THBS1
• Gene ID (NCBI): 7057
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.

Publications

Species	Application	Title
mouse	WB,IHC	Mol Med Rep; Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction.; Authors - Jin Zhao
human	WB	Oral Surg Oral Med Oral Pathol Oral Radiol; Cross-talk between synovial fibroblasts and chondrocytes in condylar hyperplasia: an in vitro pilot study.; Authors - Huilin Guo
human	WB	Int J Mol Sci; High Level of Staufen1 Expression Confers Longer Recurrence Free Survival to Non-Small Cell Lung Cancer Patients by Promoting THBS1 mRNA Degradation.; Authors - Florence Bonnet-Magnaval
human	WB	Oral Surg Oral Med Oral Pathol Oral Radiol; Upregulation of proangiogenic factors expression in the synovium of temporomandibular joint condylar hyperplasia.; Authors - Huilin Guo
human	WB	Comput Math Methods Med; miR-19a-3p Promotes Tumor-Relevant Behaviors in Bladder Urothelial Carcinoma via Targeting THBS1.; Authors - Gang Xu
human	WB	J Oral Pathol Med; IL-1β-regulating angiogenic factors expression in perforated temporomandibular disk cells via NF-κB pathway.; Authors - Jie Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Emma (Verified Customer) (03-15-2022)

A single band by WB in DU145 cells. Used at 1:1000 @ 4C overnight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Prostate

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