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Thrombospondin 1 Polyclonal antibody

Thrombospondin 1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 18304-1-AP

Synonyms

THBS, THBS 1, THBS1, thrombospondin 1, Thrombospondin-1, TSP, TSP 1, TSP1, TSP-1



Tested Applications

Positive WB detected inC6 cells, human peripheral blood platelets, HeLa cells, A431 cells, NIH/3T3 cells
Positive IHC detected inhuman lung cancer tissue, human placenta tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

18304-1-AP targets Thrombospondin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name thrombospondin 1
Calculated molecular weight 129 kDa
Observed molecular weight 180 kDa
GenBank accession numberBC028145
Gene symbol THBS1
Gene ID (NCBI) 7057
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.

Protocols

Product Specific Protocols
WB protocol for Thrombospondin 1 antibody 18304-1-APDownload protocol
IHC protocol for Thrombospondin 1 antibody 18304-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IHC

Mol Med Rep

Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction.

Authors - Jin Zhao
humanWB

Oral Surg Oral Med Oral Pathol Oral Radiol

Cross-talk between synovial fibroblasts and chondrocytes in condylar hyperplasia: an in vitro pilot study.

Authors - Huilin Guo
humanWB

Int J Mol Sci

High Level of Staufen1 Expression Confers Longer Recurrence Free Survival to Non-Small Cell Lung Cancer Patients by Promoting THBS1 mRNA Degradation.

Authors - Florence Bonnet-Magnaval
humanWB

Oral Surg Oral Med Oral Pathol Oral Radiol

Upregulation of proangiogenic factors expression in the synovium of temporomandibular joint condylar hyperplasia.

Authors - Huilin Guo
humanWB

Comput Math Methods Med

miR-19a-3p Promotes Tumor-Relevant Behaviors in Bladder Urothelial Carcinoma via Targeting THBS1.

Authors - Gang Xu
humanWB

J Oral Pathol Med

IL-1β-regulating angiogenic factors expression in perforated temporomandibular disk cells via NF-κB pathway.

Authors - Jie Xu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (03-15-2022)

A single band by WB in DU145 cells. Used at 1:1000 @ 4C overnight.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Prostate