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Thrombospondin 1 Polyclonal antibody
Thrombospondin 1 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 18304-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | C6 cells, human peripheral blood platelets, HeLa cells, A431 cells, NIH/3T3 cells |
Positive IHC detected in | human lung cancer tissue, human placenta tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 15 publications below |
IHC | See 4 publications below |
IF | See 1 publications below |
Product Information
18304-1-AP targets Thrombospondin 1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | thrombospondin 1 |
Calculated molecular weight | 129 kDa |
Observed molecular weight | 180 kDa |
GenBank accession number | BC028145 |
Gene symbol | THBS1 |
Gene ID (NCBI) | 7057 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Thrombospondin 1(TSP-1), an extracellular matrix protein, is the first identified natural angiogenesis inhibitor. A variety of normal cells, including endothelial cells, fibroblasts, adipocytes, smooth muscle cells, monocytes, macrophages, and transformed cells such as malignant glioma cells, secrete Thrombospondin 1. It regulates cellular phenotype during tissue genesis and repair. It acts as a molecular facilitator by bringing together cytokines, growth factors, matrix components, membrane receptors and extracellular proteases. Thrombospondin 1 binds to a wide variety of integrin and non-integrin cell surface receptors. It is also a major activator of transforming growth factor (TGFβ1). Secreted TSP1 is a glycoprotein with a molecular mass of 150-180 kDa.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Thrombospondin 1 antibody 18304-1-AP | Download protocol |
IHC protocol for Thrombospondin 1 antibody 18304-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Med Rep Astaxanthin ameliorates renal interstitial fibrosis and peritubular capillary rarefaction in unilateral ureteral obstruction. | ||
Oral Surg Oral Med Oral Pathol Oral Radiol Cross-talk between synovial fibroblasts and chondrocytes in condylar hyperplasia: an in vitro pilot study. | ||
Int J Mol Sci High Level of Staufen1 Expression Confers Longer Recurrence Free Survival to Non-Small Cell Lung Cancer Patients by Promoting THBS1 mRNA Degradation. | ||
Oral Surg Oral Med Oral Pathol Oral Radiol Upregulation of proangiogenic factors expression in the synovium of temporomandibular joint condylar hyperplasia. | ||
Comput Math Methods Med miR-19a-3p Promotes Tumor-Relevant Behaviors in Bladder Urothelial Carcinoma via Targeting THBS1. | ||
J Oral Pathol Med IL-1β-regulating angiogenic factors expression in perforated temporomandibular disk cells via NF-κB pathway. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Emma (Verified Customer) (03-15-2022) | A single band by WB in DU145 cells. Used at 1:1000 @ 4C overnight.
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