Anticorps Polyclonal de lapin anti-BAD

BAD Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications

93

Avis (1)

N° de cat : 10435-1-AP

Synonymes

Bcl2-L-8, BCL2L8, Bcl-2-binding component 6, Bcl2-associated agonist of cell death, BBC6

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Galerie de données de validation

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  WB analysis of Jurkat using 10435-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10435-1-AP (BAD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10435-1-AP (BAD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10435-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10435-1-AP (BAD antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse liver using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10435-1-AP (BAD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10435-1-AP (BAD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate hyperplasia using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10435-1-AP (BAD Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 10435-1-AP (BAD Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate cancer using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10435-1-AP (BAD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10435-1-AP (BAD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10435-1-AP (BAD Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lymphoma using 10435-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 10435-1-AP (BAD Antibody) at dilution of 1:200 (under 40x lens).

• 

  FC experiment of Jurkat using 10435-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human BAD (10435-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules Jurkat, cellules Neuro-2a
• Résultats positifs en IHC: tissu d'hyperplasie de la prostate humain, tissu de cancer de la prostate humain, tissu de lymphome humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:100-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 91 publications below
• IHC: See 4 publications below

Informations sur le produit

10435-1-AP cible BAD dans les applications de WB, IHC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BAD Protéine recombinante Ag0688
• Nom complet: BCL2-associated agonist of cell death
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18-25 kDa
• Numéro d’acquisition GenBank: BC001901
• Symbole du gène: BAD
• Identification du gène (NCBI): 572
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BAD, also named as BBC6 and BCL2L8, promotes cell death. It is successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. BAD can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2. It appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

Publications

Species	Application	Title
human	WB	Carbohydr Polym; A multi-bioresponsive self-assembled nano drug delivery system based on hyaluronic acid and geraniol against liver cancer; Authors - Shaofeng Duan
human	WB	Oxid Med Cell Longev; Cystathionine β-Synthase Regulates the Proliferation, Migration, and Invasion of Thyroid Carcinoma Cells.; Authors - Qi-Ying Jiang
human	WB	Oxid Med Cell Longev; Dihydroartemisinin Modulates Apoptosis and Autophagy in Multiple Myeloma through the P38/MAPK and Wnt/β-Catenin Signaling Pathways.; Authors - Xiuhua Wu
human	WB	Int J Nanomedicine; Synergism of cisplatin-oleanolic acid co-loaded calcium carbonate nanoparticles on hepatocellular carcinoma cells for enhanced apoptosis and reduced hepatotoxicity.; Authors - Muhammad Waseem Khan
human	WB	Cell Death Dis; CRB3 regulates contact inhibition by activating the Hippo pathway in mammary epithelial cells.; Authors - Xiaona Mao
human	WB	Oncotarget; Targeting ERK enhances the cytotoxic effect of the novel PI3K and mTOR dual inhibitor VS-5584 in preclinical models of pancreatic cancer.; Authors - Changwen Ning

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

• Applications: Other

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