Anticorps Monoclonal anti-4EBP1

• Phare
• Validé par KD/KO

4EBP1 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, porc, rat, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

47

CloneNo.

2C3F3

Avis (2)

N° de cat : 60246-1-Ig

Synonymes

4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I

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Galerie de données de validation

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  WB analysis of K562 using 60246-1-Ig

  WB result of 4EBP1 antibody (60246-1-Ig, 1:4000) with si-Control and si-4EBP1 transfected K562 cells.

• 

  WB analysis of K-562 using 60246-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NCI-H1299 using 60246-1-Ig

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 60246-1-Ig

  HCT 116 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 60246-1-Ig

  C6 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 60246-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 60246-1-Ig

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60246-1-Ig (4EBP1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat liver using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat pancreas using 60246-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 60246-1-Ig (4EBP1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 60246-1-Ig

  Immunofluorescent analysis of (-20℃ Ethanol ) fixed HeLa cells using 60246-1-Ig(4EBP1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules C6, cellules HCT 116, cellules NCI-H1299, cellules RAW 264.7
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du côlon humain, tissu hépatique de rat, tissu hépatique de souris, tissu pancréatique de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 42 publications below
• IHC: See 3 publications below

Informations sur le produit

60246-1-Ig cible 4EBP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, poulet
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: 4EBP1 Protéine recombinante Ag18985
• Nom complet: eukaryotic translation initiation factor 4E binding protein 1
• Masse moléculaire calculée: 118 aa, 12 kDa
• Poids moléculaire observé: 15-24 kDa
• Numéro d’acquisition GenBank: BC004459
• Symbole du gène: EIF4EBP1
• Identification du gène (NCBI): 1978
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Eukaryotic translation initiation factor 4E-binding protein 1(4EBP1), is a member of 4EBPs family, which regulate the translation of a subset of mRNA by competing with eIF4G for binding to eIF4E, thus preventing the assembly of the eIF4F complex. The eIF4F facilitates the recruitment of other translation initiation factors to form the complex and then initiates cap-dependent translation.4EBP1 also mediates the regulation of protein translation by growth factors, hormones and other stimuli that signal through the MAP kinase and mTORC1 pathways. There are three forms of 4EBP1, alpha, beta and gamma.typical pattern seen on Western blots for phosphorylated heat-acid stabled protein 4EBP1 from rat gastrocnemius muscle. Three distinct bands are noted, with the most rapidly migrating band having an apparent molecular mass of ; 20 kDa and the slowest migrating band of; 24 kDa. The 3 bands are designated by convention asalpha, beta and gamma. (PMID: 10913029)

Publications

Species	Application	Title
	WB	Nature; Feeding induces cholesterol biosynthesis via the mTORC1-USP20-HMGCR axis.; Authors - Xiao-Yi Lu
		J Hepatol; OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism.; Authors - Weiqi Dai
human	IHC	Mol Cancer; The circular RNA circ-ERBIN promotes growth and metastasis of colorectal cancer by miR-125a-5p and miR-138-5p/4EBP-1 mediated cap-independent HIF-1α translation.; Authors - Liang-Yan Chen
human	WB	Cell Death Differ; Lysine methylation of PPP1CA by the methyltransferase SUV39H2 disrupts TFEB-dependent autophagy and promotes intervertebral disc degeneration; Authors - Huaizhen Liang
human	WB	Autophagy; STYK1 promotes autophagy through enhancing the assembly of autophagy-specific class III phosphatidylinositol 3-kinase complex I.; Authors - Cefan Zhou
	WB,IHC	Toxicology; Toxicity mechanism of peri-implantation pesticide beta-cypermethrin exposure on endometrial remodeling in early pregnant mice; Authors - Yong-Jiang Zhou

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tom (Verified Customer) (03-02-2021)

10ug of HEK293 lysate resolved on 4-20% gradient acrylamide gel. Membrane blocked (5% BSA) then incubated overnight with antibody (1:2,000). Incubated with secondary HRP antibody for visulization.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HEK293

4EBP1 Antibody Western Blot validation (1:2000 dilution) in HEK293 (Cat no:60246-1-Ig)

FH

Yifei (Verified Customer) (11-01-2017)

It works well for Western blot

• Applications: Western Blot

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