WDR24 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

WDR24 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IP, IHC, IF, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Kat-Nr. : 20778-1-AP

Synonyme

C16orf21, JFP7, WD repeat domain 24, WDR24

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 20778-1-AP

  WB result of WDR24 antibody (20778-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WDR24 transfected HEK-293 cells.

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  WB analysis using 20778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20778-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20778-1-AP (WDR24 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20778-1-AP

  IP result of anti-WDR24(IP:20778-1-AP, 4ug; Detection:20778-1-AP 1:3000) with HeLa cells lysate 1560 ug.

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  IHC staining of human colon cancer using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 20778-1-AP (WDR24 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 20778-1-AP (WDR24 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 20778-1-AP (WDR24 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human colon using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20778-1-AP (WDR24 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 20778-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20778-1-AP (WDR24 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 20778-1-AP

  Immunofluorescent analysis of Hela cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  IF Staining of HepG2 using 20778-1-AP

  Immunofluorescent analysis of HepG2 cells, using WDR24 antibody 20778-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, Maushirngewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Kolonkarzinomgewebe, humanes Eierstockgewebe, humanes Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen, HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 18 publications below
• IF: See 2 publications below
• IP: See 7 publications below
• CoIP: See 1 publications below

Produktinformation

20778-1-AP bindet in WB, IP, IHC, IF, CoIP, ELISA WDR24 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: WDR24 fusion protein Ag14774
• Vollständiger Name: WD repeat domain 24
• Berechnetes Molekulargewicht: 920 aa, 102 kDa
• Beobachtetes Molekulargewicht: 102 kDa, 88 kDa
• GenBank-Zugangsnummer: BC008025
• Gene symbol: WDR24
• Gene ID (NCBI): 84219
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

WDR24 is a component of the GATOR2 complex which has five known subunits (WDR24, WDR59, Mios, Sec13, and Seh1L). GATOR2 complex positively regulates mTORC1 signaling by acting upstream of or in parallel to GATOR1, which is a GTPase-activating protein (GAP) for RagA or RagB and an inhibitor of the amino-acid-sensing pathway. (PMID: 23723238; 25263562)

Publikationen

Species	Application	Title
	WB,IP	Nature; SAR1B senses leucine levels to regulate mTORC1 signalling.; Authors - Jie Chen
human	IP,WB	Nature; CRISPR screens unveil signal hubs for nutrient licensing of T cell immunity.; Authors - Lingyun Long
human	WB,IP	Nature; Structure of the nutrient-sensing hub GATOR2.; Authors - Max L Valenstein
mouse	WB,CoIP	Science; Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.; Authors - Andrew L Cangelosi
human	WB	Nature; SZT2 dictates GATOR control of mTORC1 signalling.; Authors - Min Peng ; KO Validated
human	WB	Nat Commun; Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation; Authors - Xing Chen

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