SEC13 Polyclonal antibody

SEC13 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

16

Reviews (1)

Cat no : 15397-1-AP

Synonyms

D3S1231E, Protein SEC13 homolog, SEC13, SEC13 homolog (S. cerevisiae), SEC13 like protein 1, SEC13 related protein, SEC13L1, SEC13R

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Validation Data Gallery

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  WB analysis using 15397-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15397-1-AP (SEC13 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 15397-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 15397-1-AP (SEC13 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15397-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15397-1-AP (SEC13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 15397-1-AP

  IP result of anti-SEC13 (IP:15397-1-AP, 3ug; Detection:15397-1-AP 1:1000) with HepG2 cells lysate 3200ug.

• 

  IHC staining of mouse brain using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15397-1-AP (SEC13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse liver using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15397-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15397-1-AP (SEC13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15397-1-AP

  Immunofluorescent analysis of HepG2 cells using 15397-1-AP (SEC13 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 15397-1-AP

  Immunofluorescent analysis of HepG2 cells, using 15397-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: HepG2 cells, RAW 264.7 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: mouse brain tissue, mouse liver tissue, human kidney tissue, human placenta tissue, human testis tissue, human skin tissue, human spleen tissue, human lung tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IF: See 1 publications below

Product Information

15397-1-AP targets SEC13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: SEC13 fusion protein Ag7610
• Full Name: SEC13 homolog (S. cerevisiae)
• Calculated Molecular Weight: 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC002634
• Gene Symbol: SEC13
• Gene ID (NCBI): 6396
• RRID: AB_2186234
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SEC13 is a component of the nuclear pore complex (NPC) and the COPII coat. It is required for esicle biogenesis from endoplasmic reticulum during the transport of proteins. Shuttling of SEC13 between the nucleus and the cytoplasm may couple and regulate functions between these two compartments. (PMID: 14517296; 16407955)

Publications

Species	Application	Title
human	WB	Nature; Structure of the nutrient-sensing hub GATOR2.; Authors - Max L Valenstein
human	WB	Nat Commun; Intracellular galectin-3 is a lipopolysaccharide sensor that promotes glycolysis through mTORC1 activation; Authors - Xing Chen
		Dev Cell; Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells; Authors - Wenxiu Dai
human,mouse	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
rat	WB	Proc Natl Acad Sci U S A; TFG facilitates outer coat disassembly on COPII transport carriers to promote tethering and fusion with ER-Golgi intermediate compartments.; Authors - Michael G Hanna
human	WB	Proc Natl Acad Sci U S A; Atlastin-mediated membrane tethering is critical for cargo mobility and exit from the endoplasmic reticulum.; Authors - Liling Niu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Subir (Verified Customer) (09-27-2019)

clean and specific

• Applications: Western Blot,
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Protein from mouse tail tendon

SEC13 Antibody Western Blot, validation (1:500 dilution) in Protein from mouse tail tendon (Cat no:15397-1-AP)

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