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  • KD/KO Validated

Sestrin 2 Polyclonal antibody

Sestrin 2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB,IP,IHC,IF,ELISA

Conjugate

Unconjugated

Cat no : 10795-1-AP

Synonyms

HI95, SES2, SESN2, SEST2, Sestrin 2, Sestrin2, Sestrin-2



Tested Applications

Positive WB detected inHEK-293 cells, human liver tissue, human brain tissue, NIH/3T3 cells, rat liver tissue, HeLa cells, K-562 cells, mouse kidney tissue, A2780 cells, RAW 264.7 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse kidney tissue, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10795-1-AP targets Sestrin 2 in WB,IP,IHC,IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, Bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Sestrin 2 fusion protein Ag1247
Full Name sestrin 2
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54-60 kDa
GenBank Accession NumberBC013304
Gene Symbol SESN2
Gene ID (NCBI) 83667
RRIDAB_2185480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SESN is a family of highly conserved, stress-inducible genes that can defend the cell against oxidative damage and oncogenic signaling. SESN2 ,which belongs to the SESN family, has been implicated as a tumor suppressor that can inhibit angiogenesis and promote autophagy, underscoring the importance of elucidating the molecular mechanism by which SESN2 regulates pathways of metabolism and survival.(PMID:22363791). Sesn2 encodes a 480 amino acid protein which can run as a 60 kDa band on SDS-polyacrylamide gel electrophoresis(PMID:20878915).

Protocols

Product Specific Protocols
WB protocol for Sestrin 2 antibody 10795-1-APDownload protocol
IHC protocol for Sestrin 2 antibody 10795-1-APDownload protocol
IF protocol for Sestrin 2 antibody 10795-1-APDownload protocol
IP protocol for Sestrin 2 antibody 10795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Systematic quantitative analysis of ribosome inventory during nutrient stress.

Authors - Heeseon An
mouseWB

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
humanWB

Nature

Stem cell function and stress response are controlled by protein synthesis.

Authors - Sandra Blanco
mouseWB

Science

Zonated leucine sensing by Sestrin-mTORC1 in the liver controls the response to dietary leucine.

Authors - Andrew L Cangelosi
humanWB

Nature

SZT2 dictates GATOR control of mTORC1 signalling.

Authors - Min Peng
mouseWB

Cell

Sestrins Function as Guanine Nucleotide Dissociation Inhibitors for Rag GTPases to Control mTORC1 Signaling.

Authors - Min Peng
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ashish (Verified Customer) (01-04-2022)

This is very good antibody for western blotting , even works good for IHC

  • Applications: Western Blot, Immunohistochemistry, Immunoprecipitation
  • Primary Antibody Dilution: 1:3000
  • Cell Tissue Type: HeK293T , HeLA
FH

H (Verified Customer) (04-05-2020)

This antibody works very well and very sensitive. I always get a specific and thick band.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
Sestrin 2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:10795-1-AP)