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  • KD/KO Validated

Sestrin 2 Monoclonal antibody

Sestrin 2 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rat and More (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

CloneNo.

1A1A4

Cat no : 66297-1-Ig

Synonyms

HI95, SES2, SESN2, SEST2, sestrin 2, Sestrin2



Tested Applications

Positive WB detected inHeLa cells, HEK-293T
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66297-1-Ig targets Sestrin 2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, pig, rat samples.

Tested Reactivity human, pig, rat
Cited Reactivityhuman, mouse, rat, pig, bovine
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Sestrin 2 fusion protein Ag20638
Full Name sestrin 2
Calculated Molecular Weight 54 kDa
Observed Molecular Weight 54 kDa
GenBank Accession NumberBC013304
Gene Symbol SESN2
Gene ID (NCBI) 83667
RRIDAB_2881680
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).

Protocols

Product Specific Protocols
WB protocol for Sestrin 2 antibody 66297-1-IgDownload protocol
IHC protocol for Sestrin 2 antibody 66297-1-IgDownload protocol
IF protocol for Sestrin 2 antibody 66297-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IF

Cell Biol Toxicol

Induction of Sestrin2 by pterostilbene suppresses ethanol-triggered hepatocyte senescence by degrading CCN1 via p62-dependent selective autophagy.

Authors - Yiming Jiang
  • KD Validated
humanWB,IHC

Osteoarthritis Cartilage

Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.

Authors - T Shen
pigWB, IP

J Nutr

Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.

Authors - Agus Suryawan
human,mouseWB,IHC,IF,IP

Cell Tissue Res

Sestrin2 attenuates renal damage by regulating Hippo pathway in diabetic nephropathy.

Authors - Yawei Bian
pigWB

Am J Physiol Endocrinol Metab

Prematurity blunts the insulin- and amino acid-induced stimulation of translation initiation and protein synthesis in skeletal muscle of neonatal pigs.

Authors - Marko Rudar
mouseWB

Arch Pharm Res

Sestrin2 induction contributes to anti-inflammatory responses and cell survival by globular adiponectin in macrophages

Authors - Sumin Lee
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Yahir Alberto (Verified Customer) (11-12-2021)

This antibody works nicely for western blotting, although with several unspecific bands for a monoclonal antibody.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK 293T cells
Sestrin 2 Antibody Western Blot validation (1:1000 dilution) in HEK 293T cells (Cat no:66297-1-Ig)