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Sestrin 2 Monoclonal antibody
Sestrin 2 Monoclonal Antibody for IF, IHC, WB,ELISA
Cat no : 66297-1-Ig
Validation Data Gallery
|Positive WB detected in||HeLa cells, HEK-293T|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
66297-1-Ig targets Sestrin 2 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, pig, rat samples.
|Tested Reactivity||human, pig, rat|
|Cited Reactivity||human, mouse, rat, bovine, pig|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Sestrin 2 fusion protein Ag20638|
|Full Name||sestrin 2|
|Calculated molecular weight||54 kDa|
|Observed molecular weight||54 kDa|
|GenBank accession number||BC013304|
|Gene ID (NCBI)||83667|
|Purification Method||Protein G purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids).
Cell Biol Toxicol
Induction of Sestrin2 by pterostilbene suppresses ethanol-triggered hepatocyte senescence by degrading CCN1 via p62-dependent selective autophagy.
Suppression of Sestrins in aging and osteoarthritic cartilage: dysfunction of an important stress defense mechanism.
Intermittent Bolus Compared With Continuous Feeding Enhances Insulin and Amino Acid Signaling to Translation Initiation in Skeletal Muscle of Neonatal Pigs.
Cell Tissue Res
Sestrin2 attenuates renal damage by regulating Hippo pathway in diabetic nephropathy.
Am J Physiol Endocrinol Metab
Prematurity blunts the insulin- and amino acid-induced stimulation of translation initiation and protein synthesis in skeletal muscle of neonatal pigs.
Arch Pharm Res
Sestrin2 induction contributes to anti-inflammatory responses and cell survival by globular adiponectin in macrophages
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Yahir Alberto (Verified Customer) (11-12-2021)
This antibody works nicely for western blotting, although with several unspecific bands for a monoclonal antibody.