TORC1/CRTC1 Polyclonal antibody

• Featured Product
• KD/KO Validated

TORC1/CRTC1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 10441-1-AP

Synonyms

CRTC1, KIAA0616, MECT1, TORC 1, TORC1, TORC1/CRTC1, Transducer of CREB protein 1, WAMTP1

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Validation Data Gallery

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  WB analysis of mouse kidney using 10441-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10441-1-AP (TORC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 10441-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 10441-1-AP (TORC1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10441-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10441-1-AP (TORC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10441-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10441-1-AP (TORC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 10441-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 10441-1-AP (TORC1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse kidney using 10441-1-AP

  IP result of anti-TORC1/CRTC1 (IP:10441-1-AP, 4ug; Detection:10441-1-AP 1:800) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human colon cancer using 10441-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10441-1-AP (TORC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10441-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10441-1-AP (TORC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10441-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10441-1-AP (TORC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10441-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10441-1-AP (TORC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, A431 cells, Jurkat cells, L02 cells, mouse brain tissue
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human colon cancer tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

10441-1-AP targets TORC1/CRTC1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TORC1/CRTC1 fusion protein Ag0693
• Full Name: CREB regulated transcription coactivator 1
• Calculated Molecular Weight: 75 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC028050
• Gene Symbol: CRTC1
• Gene ID (NCBI): 23373
• RRID: AB_2083835
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CRTC1, also named as MECT1,TORC1, and WAMTP1, belongs to the TORC family. It is a transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. CRTC1 acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. It enhances the interaction of CREB1 with TAF4. CRTC1 regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. It is a potent coactivator of PGC1alpha and inducer of mitochondrial biogenesis in muscle cells. CRTC1 is also a coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR). In the hippocampus, CRTC1 involved in late-phase long-term potentiation (L-LTP) maintenance at the Schaffer collateral-CA1 synapses. It may be required for dendritic growth of developing cortical neurons. This is a rabbit polyclonal antibody raised against residues near N terminus of human CRTC1. CRTC1 has some isoforms produced by alternative splicing with MW of 67 kDa, 68 kDa and 62 kDa. Sometimes it appears as a 75-80 kDa band probably due to phosphorylation (PMID: 17183642; 22770221)

Publications

Species	Application	Title
human	WB	Nat Cell Biol; Eosinophil extracellular traps drive asthma progression through neuro-immune signals.; Authors - Yiwen Lu ; KD Validated
mouse	WB,IP	iScience; Neural and central mechanisms of kidney fibrosis after relief of ureteral obstruction; Authors - Huanjuan Su
	IHC	J Ethnopharmacol; Exploring the potential mechanism of Heng-Gu-Gu-Shang-Yu-He-Ji therapy for osteoporosis based on network pharmacology and transcriptomics; Authors - Linbi Xie
mouse	WB	Sci Rep; Targeted Metabolomics Reveals Abnormal Hepatic Energy Metabolism by Depletion of β-Carotene Oxygenase 2 in Mice.; Authors - Lei Wu
		Ann N Y Acad Sci; PMID: 26645136; Authors - Holly M Brown-Borg
rat	WB,IF	J Agric Food Chem; Fluoride Stimulates Anxiety- and Depression-like Behaviors Associated with SIK2-CRTC1 Signaling Dysfunction.; Authors - Guoyu Zhou