VEGFC Polyklonaler Antikörper

VEGFC Polyklonal Antikörper für IF, IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

28

Rezensionen (2)

Kat-Nr. : 22601-1-AP

Synonyme

Flt4 L, Flt4 ligand, VEGF C, VEGFC, VRP

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Galerie der Validierungsdaten

• 

  WB analysis using 22601-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22601-1-AP (VEGFC antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 22601-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22601-1-AP (VEGFC antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW 264.7 using 22601-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 22601-1-AP (VEGFC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 22601-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22601-1-AP (VEGFC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22601-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22601-1-AP (VEGFC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 22601-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22601-1-AP (VEGFC Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 22601-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22601-1-AP (VEGFC Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human lung cancer using 22601-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22601-1-AP (VEGFC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human lung cancer using 22601-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 22601-1-AP (VEGFC Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of RAW 264.7 using 22601-1-AP

  Immunofluorescent analysis of RAW 264.7 cells using 22601-1-AP (VEGFC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF-7-Zellen, HCT 116-Zellen, RAW 264.7-Zellen, SW480-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe, humanes Lungenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: RAW 264.7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 20 publications below
• IHC: See 12 publications below
• IF: See 4 publications below

Produktinformation

22601-1-AP bindet in WB, IHC, IF, ELISA VEGFC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: VEGFC fusion protein Ag18182
• Vollständiger Name: vascular endothelial growth factor C
• Berechnetes Molekulargewicht: 419 aa, 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC035212
• Gene symbol: VEGFC
• Gene ID (NCBI): 7424
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

VEGFC is a member of the platelet-derived growth factor / vascular endothelial growth factor (PDGF/VEGF) family. The main function of VEGFC is to promote the growth of lymphatic vessels (lymphangiogenesis). It acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth, and migration.

Publikationen

Species	Application	Title
mouse	WB,IHC	J Extracell Vesicles; DUSP2 regulates extracellular vesicle-VEGF-C secretion and pancreatic cancer early dissemination.; Authors - Chu-An Wang
mouse	WB	Clin Transl Med; VEGF-C/VEGFR-3 axis protects against pressure-overload induced cardiac dysfunction through regulation of lymphangiogenesis.; Authors - Qiu-Yue Lin
human	WB, IHC, IF	Cancer Lett; The Hippo-TAZ axis mediates vascular endothelial growth factor C in glioblastoma-derived exosomes to promote angiogenesis.; Authors - Zhengmin Wang
mouse	WB	Oxid Med Cell Longev; Angiotensin II Induces Cardiac Edema and Hypertrophic Remodeling through Lymphatic-Dependent Mechanisms.; Authors - Jie Bai
mouse	IHC	Front Pharmacol; Fenofibrate suppresses corneal neovascularization by regulating lipid metabolism through PPARα signaling pathway; Authors - Tong Zhou
human	WB	Aging (Albany NY); MicroRNA-330-3p promotes brain metastasis and epithelial-mesenchymal transition via GRIA3 in non-small cell lung cancer.; Authors - Chunhua Wei

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guorong (Verified Customer) (11-22-2022)

No bands were observed. Does not represent the performance in other kind of samples

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: ARPE-19

FH

hongxuan (Verified Customer) (11-12-2018)

we can detect the obvious signals in the embryo sections by using this primary antibody at a dilution of 1:50. Moreover, the background is very clear. Please forgive me that I cannot upload the files due to it's unpublished data.Thank you very much for you product.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: mouse embryo

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