TMEM173/STING Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

TMEM173/STING Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (2)

Anwendung

WB, IP, IHC, IF, FC, CoIP, Blocking assay, ELISA

Konjugation

Unkonjugiert

Publikationen

238

Rezensionen (1)

Kat-Nr. : 19851-1-AP

Synonyme

hMITA, hSTING, Mediator of IRF3 activation, MITA, MPYS, NET23, STING, STING1, TMEM173, TMEM173/STING, transmembrane protein 173

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Galerie der Validierungsdaten

• 

  WB analysis using 19851-1-AP

  Various lysis were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 19851-1-AP

  WB result of TMEM173/STING antibody (19851-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected THP-1 cells.

• 

  WB analysis of HepG2 using 19851-1-AP

  WB result of TMEM173/STING antibody (19851-1-AP; 1:6000; room temperature for 1.5 hours) with wild-type and TMEM173/STING knockout HepG2 cells.

• 

  WB analysis using 19851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 19851-1-AP

  WB result of TMEM173/STING antibody (19851-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM173/STING transfected HEK-293 cells.

• 

  WB analysis of rat heart using 19851-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 19851-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 19851-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 19851-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 19851-1-AP (TMEM173/STING antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 19851-1-AP

  IP Result of anti-TMEM173/STING (IP:19851-1-AP, 4ug; Detection:19851-1-AP 1:1000) with mouse spleen tissue lysate 4000ug.

• 

  IHC staining of human tonsillitis using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse thymus using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:2000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 19851-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 19851-1-AP (TMEM173/STING antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HT-29 using 19851-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of human tonsillitis using 19851-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 19851-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using TMEM173/STING antibody (19851-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of THP-1 using 19851-1-AP

  1X10^6 THP-1 cells were intracellularly stained with 0.4 ug Anti-Human TMEM173/STING (19851-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, C2C12-Zellen, HepG2-Zellen, HL-60-Zellen, HT-29-Zellen, Maushodengewebe, Mausmilzgewebe, Mausnierengewebe, Maus-Thymusgewebe, Raji-Zellen, THP-1-Zellen
• Erfolgreiche IP: Mausmilzgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Leberkarzinomgewebe, humanes Milzgewebe, Maus-Thymusgewebe, Ratten-Thymusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HT-29-Zellen, humanes Tonsillitisgewebe
• Erfolgreiche Detektion in FC: THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:2000-1:8000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 42 publications below
• WB: See 195 publications below
• IHC: See 40 publications below
• IF: See 75 publications below
• IP: See 10 publications below
• FC: See 3 publications below
• CoIP: See 5 publications below

Produktinformation

19851-1-AP bindet in WB, IP, IHC, IF, FC, CoIP, Blocking assay, ELISA TMEM173/STING und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Affe, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: TMEM173/STING fusion protein Ag13921
• Vollständiger Name: transmembrane protein 173
• Berechnetes Molekulargewicht: 379 aa, 42 kDa
• Beobachtetes Molekulargewicht: 35-40 kDa, 80 kDa
• GenBank-Zugangsnummer: BC047779
• Gene symbol: TMEM173
• Gene ID (NCBI): 340061
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Stimulator of interferon genes (STING, also known as ERIS, MITA and MPYS, and encoded by TMEM173) is a transmembrane adaptor protein that facilitates innate immune signaling (PMID: 18724357). STING is widely expressed in various cell types such as endothelial cells, epithelial cells, T cells, macrophages, and dendritic cells (PMID: 26603901). It is predominantly located in the endoplasmic reticulum (ER). STING functions as a sensor of cytosolic DNA and promotes the production of type I interferons and pro-inflammatory cytokines. STING is a 379 amino acid protein with a calculated molecular weight of 42 kDa. It has been observed at 35-40 kDa (PMID: 27324217; 29632140; 30918080), and 70-80 kDa corresponding to the expected size of a STING dimer (PMID: 25790474; 29491158).

Publikationen

Species	Application	Title
mouse	IF	Nature; Tonic prime-boost of STING signalling mediates Niemann-Pick disease type C.; Authors - Ting-Ting Chu ; KO Validated
mouse	WB	Nature; eccDNAs are apoptotic products with high innate immunostimulatory activity; Authors - Yuangao Wang ; KO Validated
human	WB	Science; The heme-regulated inhibitor is a cytosolic sensor of protein misfolding that controls innate immune signaling.; Authors - Mena Abdel-Nour
human	WB,IF	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng ; KD Validated
human	WB	Cancer Cell; Mutant p53 suppresses innate immune signaling to promote tumorigenesis.; Authors - Monisankar Ghosh ; KD Validated
mouse	IF	Nat Immunol; Homeostatic regulation of STING protein at the resting state by stabilizer TOLLIP.; Authors - Vladislav Pokatayev

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Will (Verified Customer) (04-25-2019)

good antibody, will order again

• Applications: Western Blot,

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