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  • KD/KO Validated

IRF3 Polyclonal antibody

IRF3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse and More (4)





Cat no : 11312-1-AP


interferon regulatory factor 3, IRF 3, IRF3

Tested Applications

Positive WB detected inHEK-293T cells, U-937 cells, human brain tissue, mouse brain tissue, Jurkat cells, HeLa cells, MCF-7 cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite®488 and CoraLite®555 conjugated versions of this antibody.

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11312-1-AP targets IRF3 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, monkey, pig, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF3 fusion protein Ag1858
Full Name interferon regulatory factor 3
Calculated molecular weight 47 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC009395
Gene symbol IRF3
Gene ID (NCBI) 3661
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. IRF3 exists some isoforms with MV 47-49 kDa, 33 kDa and 12-16 kDa.


Product Specific Protocols
WB protocol for IRF3 antibody 11312-1-APDownload protocol
IHC protocol for IRF3 antibody 11312-1-APDownload protocol
IF protocol for IRF3 antibody 11312-1-APDownload protocol
IP protocol for IRF3 antibody 11312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Front Immunol

Herpes Simplex Virus Type 2 Immediate Early Protein ICP27 Inhibits IFN-β Production in Mucosal Epithelial Cells by Antagonizing IRF3 Activation.

Authors - Xinmeng Guan

Front Physiol

Evidence for Sexual Dimorphism in the Response to TLR3 Activation in the Developing Neonatal Mouse Brain: A Pilot Study.

Authors - Raul Chavez-Valdez


USP7-TRIM27 axis negatively modulates antiviral type I IFN signaling.

Authors - Juan Cai

ACS Infect Dis

PMID: 32167734

Authors - Yuan-Qin Min

Virol Sin

SRP54 Negatively Regulates IFN-Beta Production and Antiviral Response by Targeting RIG-I and MDA5.

Authors - Dong-Peng Wang

Cell Rep

A Nuclear Export Signal Is Required for cGAS to Sense Cytosolic DNA.

Authors - Hong Sun


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sarah (Verified Customer) (12-18-2019)

This antibody is awesome- been searching for an Pab IRF 3 antibody that works well in a species other than human, and we finally found this one! Thank you!

  • Applications: Western Blot,