MAVS; VISA Polyclonal antibody

• Featured Product
• KD/KO Validated

MAVS; VISA Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

64

Reviews (1)

Cat no : 14341-1-AP

Synonyms

MAVS, KIAA1271, IPS-1, IPS1, IPS 1

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Validation Data Gallery

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  WB analysis using 14341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14341-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 14341-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14341-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 14341-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14341-1-AP (MAVS; VISA Antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 14341-1-AP

  IP result of anti-MAVS; VISA (IP:14341-1-AP, 3ug; Detection:14341-1-AP 1:1000) with HEK-293 cells lysate 1700ug.

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  IHC staining of human breast cancer using 14341-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14341-1-AP (MAVS; VISA antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14341-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14341-1-AP (MAVS; VISA antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14341-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14341-1-AP (MAVS; VISA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 14341-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14341-1-AP (MAVS; VISA Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human skin using 14341-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 14341-1-AP (MAVS; VISA Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 14341-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 14341-1-AP (MAVS; VISA antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 14341-1-AP

  Immunofluorescent analysis of Hela cells, using MAVS antibody 14341-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: A431 cells, Jurkat cells, HeLa cells, HuH-7 cells, HepG2 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 12 publications below
• WB: See 59 publications below
• IF: See 8 publications below
• IP: See 5 publications below

Product Information

14341-1-AP targets MAVS; VISA in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAVS; VISA fusion protein Ag5655
• Full Name: mitochondrial antiviral signaling protein
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 50-55 kDa, 70-75 kDa
• GenBank Accession Number: BC044952
• Gene Symbol: MAVS
• Gene ID (NCBI): 57506
• RRID: AB_10548408
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mitochondrial antiviral-signaling protein (MAVS) is also known as virus-induced-signaling adapter (VISA) or IFN-β promoter stimulator protein 1 (IPS-1), it is widely involved and required for innate immune defense against viruses. MAVS, present in T cells, monocytes, epithelial cells and hepatocytes, contains CARD and transmembrane domains which are essential for antiviral functions. MAVS is able to interact with various cellular proteins including DDX58/RIG-I, IFIH1/MDA5, TRAF2, TRAF6, TMEM173/MITA, IFIT3 and etc. It can undergoe phosphorylation on multiple sites and ubiquitination, which may together cause the molecular weight migrate to about 70 kDa despite the predicated 57 kDa.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
		Immunity; Decreased Expression of the Host Long-Noncoding RNA-GM Facilitates Viral Escape by Inhibiting the Kinase activity TBK1 via S-glutathionylation.; Authors - Yujia Wang
	WB	Mol Cell; An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation; Authors - Stephanie Pei Tung Yiu
human	WB	Nat Commun; MLL5 suppresses antiviral innate immune response by facilitating STUB1-mediated RIG-I degradation.; Authors - Peipei Zhou
human	WB	EMBO J; CircPVT1 promotes ER-positive breast tumorigenesis and drug resistance by targeting ESR1 and MAVS; Authors - Jia Yi ; KD Validated
pig	WB	Autophagy; Induction of autophagy and suppression of type I IFN secretion by CSFV.; Authors - Baoming Xie

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Damien (Verified Customer) (09-03-2019)

No convince by this anti-MAVS antibody.In fact, in human cells MAVS protein is expressed in two differents forms : short-MAVS (55kDa) and full-length MAVS (72 kDa). So I have to observe two bands by WB. However, after using different conditions with this antibody according to the manufacturer's protocol I always observe a smear of protein, and one band at 72 kDa. The expected on around 55 kDa never appear. Note that my cells lysates were not degraded since the others proteins that I want to reveal were great.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: HeLa

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