RIG-1/DDX58 Polyclonal antibody

• Featured Product
• KD/KO Validated

RIG-1/DDX58 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 20566-1-AP

Synonyms

DDX58, DEAD box protein 58, RIG 1, RIG I, RIG-I

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Validation Data Gallery

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  WB analysis using 20566-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20566-1-AP (RIG-1/DDX58 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 20566-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20566-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20566-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20566-1-AP (DDX58 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 20566-1-AP

  IP Result of anti-DDX58 (IP:20566-1-AP, 4ug; Detection:20566-1-AP 1:300) with HepG2 cells lysate 3600ug.

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  IF Staining of HepG2 using 20566-1-AP

  Immunofluorescent analysis of HepG2 cells, using DDX58 antibody 20566-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Tested Applications

• Positive WB detected in: Jurkat cells, MCF-7 cells, mouse brain tissue, HepG2 cells, MOLT-4 cells
• Positive IP detected in: HepG2 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• RIP: See 1 publications below

Product Information

20566-1-AP targets RIG-1/DDX58 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: DEAD (Asp-Glu-Ala-Asp) box polypeptide 58
• Calculated molecular weight: 107 kDa
• Observed molecular weight: 101/106 kDa
• GenBank accession number: NM_014314
• Gene symbol: DDX58
• Gene ID (NCBI): 23586
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.

Publications

Species	Application	Title
human	WB	Nature; FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing.; Authors - Mathieu Ringeard
human	WB	Cell Rep; The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses.; Authors - Kerong Wang
human	WB, RIP	Cell Rep; METTL3 regulates viral m6A RNA modification and host cell innate immune responses during SARS-CoV-2 infection.; Authors - Na Li
mouse	WB	Cell Rep; Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation.; Authors - Yong Shen
human	WB	J Gen Virol; Respiratory Syncytial Virus Infection is Inhibited by Interferon-Induced Transmembrane Proteins.; Authors - Wei Zhang
mouse	WB	Int Immunopharmacol; The natural compound Cirsitakaoside enhances antiviral innate responses against vesicular stomatitis virus in vitro and in vivo.; Authors - Qianqian Di