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RIG-1/DDX58 Polyclonal antibody
RIG-1/DDX58 Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, RIP, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 20566-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, MCF-7 cells, mouse brain tissue, HepG2 cells, MOLT-4 cells |
Positive IP detected in | HepG2 cells |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 18 publications below |
IHC | See 1 publications below |
IP | See 1 publications below |
RIP | See 1 publications below |
Product Information
20566-1-AP targets RIG-1/DDX58 in WB, RIP, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 |
Calculated molecular weight | 107 kDa |
Observed molecular weight | 101/106 kDa |
GenBank accession number | NM_014314 |
Gene symbol | DDX58 |
Gene ID (NCBI) | 23586 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
DDX58, also named as RIG-1, belongs to the helicase family. It is involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). It is essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. The antibody is specific to DDX58.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for RIG-1/DDX58 antibody 20566-1-AP | Download protocol |
IF protocol for RIG-1/DDX58 antibody 20566-1-AP | Download protocol |
IP protocol for RIG-1/DDX58 antibody 20566-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature FTSJ3 is an RNA 2'-O-methyltransferase recruited by HIV to avoid innate immune sensing. | ||
Cell Rep The Glycoprotein and Nucleocapsid Protein of Hantaviruses Manipulate Autophagy Flux to Restrain Host Innate Immune Responses. | ||
Cell Rep METTL3 regulates viral m6A RNA modification and host cell innate immune responses during SARS-CoV-2 infection. | ||
Cell Rep Riok3 inhibits the antiviral immune response by facilitating TRIM40-mediated RIG-I and MDA5 degradation. | ||
J Gen Virol Respiratory Syncytial Virus Infection is Inhibited by Interferon-Induced Transmembrane Proteins. | ||
Int Immunopharmacol The natural compound Cirsitakaoside enhances antiviral innate responses against vesicular stomatitis virus in vitro and in vivo. |