IFIT2 Polyclonal antibody

• Featured Product
• KD/KO Validated

IFIT2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

43

Cat no : 12604-1-AP

Synonyms

IFI 54K, IFI 54, GARG 39, G10P2, CIG-42

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Validation Data Gallery

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  WB analysis of A431 cells using 12604-1-AP

  WB result of IFIT2 antibody (12604-1-AP, 1:300) with si-IFIT2 and si-Control transfected A431 cells.

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  WB analysis using 12604-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12604-1-AP (IFIT2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of RAW 264.7 using 12604-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 12604-1-AP (IFIT2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of A431 using 12604-1-AP

  IP result of anti-IFIT2 (IP:12604-1-AP, 4ug; Detection:12604-1-AP 1:3000) with A431 cells lysate 1040 ug.

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  IHC staining of human kidney using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12604-1-AP (IFIT2 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skin cancer using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 12604-1-AP (IFIT2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 12604-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 12604-1-AP (IFIT2 Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 12604-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using IFIT2 antibody (12604-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: A431 cells, RAW 264.7 cells, THP-1 cells
• Positive IP detected in: A431 cells
• Positive IHC detected in: human skin cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 41 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

12604-1-AP targets IFIT2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IFIT2 fusion protein Ag3283
• Full Name: IFIT 2
• Calculated Molecular Weight: 484 aa, 56 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC032839
• Gene Symbol: IFIT2
• Gene ID (NCBI): 3433
• RRID: AB_2864734
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IFIT2 belongs to the IFIT family. It contains 6 TPR repeats. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT proteins are produced in human body that are supposed to confer immunity against viral infection. These proteins are generally produced during viral infection, IFN treatment, and during pathogen recognition (Pathogen associated molecular pattern recognition) by immune system during infections.

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther; TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion; Authors - Wenwen Zheng
human	WB	Nat Immunol; A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.; Authors - Ying Kai Chan
human	WB	Mol Cell; Translation stress and collided ribosomes are co-activators of cGAS.; Authors - Li Wan
human	WB	Nat Microbiol; ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity.; Authors - GuanQun Liu
human	WB	Nat Microbiol; Influenza virus repurposes the antiviral protein IFIT2 to promote translation of viral mRNAs.; Authors - Vy Tran
		Nucleic Acids Res; IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA.; Authors - Renata C Fleith

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