Validation Data Gallery
|Positive WB detected in||A431 cells, RAW 264.7 cells|
|Positive IP detected in||mouse skin tissue|
|Positive IHC detected in||human skin cancer tissue, human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
12604-1-AP targets IFIT2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IFIT2 fusion protein Ag3283|
|Full Name||IFIT 2|
|Calculated molecular weight||484 aa, 56 kDa|
|Observed molecular weight||50-55 kDa|
|GenBank accession number||BC032839|
|Gene ID (NCBI)||3433|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
IFIT2 belongs to the IFIT family. It contains 6 TPR repeats. IFIT genes comprise a large family with three (Ifit1, Ifit2, and Ifit3) and four (IFIT1, IFIT2, IFIT3, and IFIT5) members in mice and humans, respectively. IFIT proteins are produced in human body that are supposed to confer immunity against viral infection. These proteins are generally produced during viral infection, IFN treatment, and during pathogen recognition (Pathogen associated molecular pattern recognition) by immune system during infections.
|Product Specific Protocols|
|WB protocol for IFIT2 antibody 12604-1-AP||Download protocol|
|IHC protocol for IFIT2 antibody 12604-1-AP||Download protocol|
|IF protocol for IFIT2 antibody 12604-1-AP||Download protocol|
|IP protocol for IFIT2 antibody 12604-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Signal Transduct Target Ther
TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion
A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity.
Translation stress and collided ribosomes are co-activators of cGAS.
ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity.
Influenza virus repurposes the antiviral protein IFIT2 to promote translation of viral mRNAs.
Nucleic Acids Res
IFIT3 and IFIT2/3 promote IFIT1-mediated translation inhibition by enhancing binding to non-self RNA.