SNAP25 Monoklonaler Antikörper

SNAP25 Monoklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

8

CloneNo.

3E4B7

Kat-Nr. : 60159-1-Ig

Synonyme

RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein

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Galerie der Validierungsdaten

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  WB analysis of pig brain using 60159-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 60159-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 60159-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 60159-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-12 using 60159-1-Ig

  PC-12 cells were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 60159-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 60159-1-Ig (SNAP25 antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 60159-1-Ig

  IP Result of anti-SNAP25 (IP:60159-1-Ig, 3ug; Detection:60159-1-Ig 1:500) with mouse brain tissue lysate 3600ug.

• 

  IHC staining of rat brain using 60159-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 60159-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60159-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 60159-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 60159-1-Ig (SNAP25 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-12 using 60159-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (60159-1-Ig, Clone: 3E4B7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of PC-12 using 60159-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using SNAP25 antibody (60159-1-Ig, Clone: 3E4B7 ) at dilution of 1:1000 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), SNAP25 antibody (14903-1-AP, green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Hausschwein-Hirngewebe, fetales humanes Hirngewebe, HEK-293-Zellen, Maushirngewebe, PC-12-Zellen, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Rattenhirngewebe, Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: PC-12-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:20000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:1000-1:4000
• Immunfluoreszenz (IF): IF : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Produktinformation

60159-1-Ig bindet in WB, IP, IHC, IF, ELISA SNAP25 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: SNAP25 fusion protein Ag6695
• Vollständiger Name: synaptosomal-associated protein, 25kDa
• Berechnetes Molekulargewicht: 23 kDa
• Beobachtetes Molekulargewicht: 25-30 kDa
• GenBank-Zugangsnummer: BC010647
• Gene symbol: SNAP25
• Gene ID (NCBI): 6616
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.

Publikationen

Species	Application	Title
human	IF	J Extracell Vesicles; Identification of the SNARE complex that mediates the fusion of multivesicular bodies with the plasma membrane in exosome secretion; Authors - Chuqi Liu
mouse	WB	Neuropsychopharmacology; Autism risk gene KMT5B deficiency in prefrontal cortex induces synaptic dysfunction and social deficits via alterations of DNA repair and gene transcription.; Authors - Zi-Jun Wang
mouse	WB	Biochim Biophys Acta Mol Basis Dis; Mice with the Rab10 T73V mutation exhibit anxiety-like behavior and alteration of neuronal functions in the striatum; Authors - Jing Zhang
rat	WB,IHC	Neuroscience; SNAP25/syntaxin4/VAMP2/Munc18-1 Complexes in Spinal Dorsal Horn Contributed to Inflammatory Pain.; Authors - Xing-Lian Duan
human	WB,IF	Brain Commun; Transcriptomic analysis of human brains with Alzheimer's disease reveals the altered expression of synaptic genes linked to cognitive deficits.; Authors - Jamal B Williams
		CNS Neurol Disord Drug Targets; PMID: 30318007; Authors - Fengjin Hao

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