• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SNAP25 Polyclonal antibody

SNAP25 Polyclonal Antibody for ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

ELISA

Conjugate

Unconjugated

Cat no : 10007-1-AP

Synonyms

RIC 4, RIC4, SEC9, SNAP, SNAP 25, SNAP25, SUP, Super protein

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse brain tissue using SNAP25 Polyclonal antibody (10007-1-AP)

    WB analysis of mouse brain using 10007-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10007-1-AP (SNAP25 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SNAP25 Polyclonal antibody (10007-1-AP)

    WB analysis of mouse brain using 10007-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10007-1-AP (SNAP25 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Product Information

10007-1-AP targets SNAP25 in ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Recombinant Protein
Full Name synaptosomal-associated protein, 25kDa
Calculated Molecular Weight 23 kDa
GenBank Accession NumberBC010647
Gene Symbol SNAP25
Gene ID (NCBI) 6616
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The synaptosomal associated protein of 25 kD (SNAP-25) was first identified as a major synaptic protein by Wilson and colleagues. The protein interacts with syntaxin and synaptobrevin through its N-terminal and C-terminal -helical domains. Its palmitoylation domain is located in the middle of the molecule that contains four cysteine residues. Mutation of the cysteines abolishes palmitoylation and membrane binding. Several elegant studies using synaptosome preparations and permeabilized PC12 cells have suggested that SNAP-25 may act in the late post-docking steps of exocytosis. By limited proteolysis and in vitro binding assay, it is proposed that the two helix domains act independently and contribute equally to form the SNARE complex with syntaxin and synaptobrevin. It seems that a major regulatory element is located in the C-terminus of SNAP-25. Removing a 9 amino acid sequence of SNAP-25 inhibited neurosecretion in chromaffin cells.

Protocols

Product Specific Protocols
WB protocol for SNAP25 antibody 10007-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols