Synaptotagmin-1 Polyclonal antibody

• Featured Product
• KD/KO Validated

Synaptotagmin-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 14511-1-AP

Synonyms

Synaptotagmin 1, SYT1, SYT, synaptotagmin I, SVP65

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Validation Data Gallery

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  WB analysis using 14511-1-AP

  mouse cerebellum tissue was subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HCT 116 using 14511-1-AP

  HCT 116 (WT and SYT1 KO) lysates prepared with RIPA buffer, 50 μg protein loaded. 14511-1-AP incubated at 1:500 at 4°C overnight in 5% BSA in TBST. Ponceau stained transfers shown on right. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of human liver using 14511-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 14511-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 14511-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HCT 116 using 14511-1-AP

  HCT 116 lysates prepared and IP of SYT1 performed using 1.0 μg of 14511-1-AP coupled to protein A- Sepharose beads. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

• 

  IP experiment of mouse testis using 14511-1-AP

  IP result of anti-Synaptotagmin-1 (IP:14511-1-AP, 4ug; Detection:14511-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of rat small intestine using 14511-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 14511-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 14511-1-AP (Synaptotagmin-1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HCT 116 using 14511-1-AP

  HCT 116 WT cells (yellow outline) and SYT1 KO cells (red outline) labelled with a green or a far red fluorescence dye, respectively. Cells fixed with 4% PFA and stained with 14511-1-AP at 1:500 plus DAPI. Bars = 10 μm. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency.

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, HCT 116 cells, mouse testis tissue, human liver tissue, Y79 cells
• Positive IP detected in: mouse testis tissue, HCT 116 cells
• Positive IHC detected in: rat small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HCT 116 cells

Recommended dilution

• Western Blot (WB): WB : 1:4000-1:20000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

14511-1-AP targets Synaptotagmin-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Synaptotagmin-1 fusion protein Ag5970
• Full Name: synaptotagmin I
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 65 kDa, 47 kDa
• GenBank Accession Number: BC058917
• Gene Symbol: Synaptotagmin-1
• Gene ID (NCBI): 6857
• RRID: AB_2199166
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.

Publications

Species	Application	Title
human	WB	Cell Rep; The complement inhibitor CD59 is required for GABAergic synaptic transmission in the dentate gyrus; Authors - Lang Wen
human	WB	Theranostics; ANKRD22, a novel tumor microenvironment-induced mitochondrial protein promotes metabolic reprogramming of colorectal cancer cells.; Authors - Tianhui Pan
		Front Mol Neurosci; A Stimulation Function of Synaptotagmin-1 in Ternary SNARE Complex Formation Dependent on Munc18 and Munc13.; Authors - Yun Li
mouse	IHC,WB	Biochim Biophys Acta Mol Cell Biol Lipids; Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet.; Authors - Sara Sancho-Knapik
mouse	WB	J Proteomics; Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome.; Authors - Hao-Long Zeng
rat	WB, IF	PLoS One; Identification of Protein Network Alterations upon Retinal Ischemia-Reperfusion Injury by Quantitative Proteomics Using a Rattus norvegicus Model.; Authors - Han Tian