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Synaptotagmin-1 Polyclonal antibody
Synaptotagmin-1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 14511-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse cerebellum tissue, HCT 116 cells, mouse testis tissue, human liver tissue, Y79 cells |
Positive IP detected in | mouse testis tissue, HCT 116 cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells, HCT 116 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:4000-1:20000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 14 publications below |
IHC | See 3 publications below |
IF | See 4 publications below |
Product Information
14511-1-AP targets Synaptotagmin-1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Synaptotagmin-1 fusion protein Ag5970 |
Full Name | synaptotagmin I |
Calculated Molecular Weight | 48 kDa |
Observed Molecular Weight | 65 kDa, 47 kDa |
GenBank Accession Number | BC058917 |
Gene Symbol | Synaptotagmin-1 |
Gene ID (NCBI) | 6857 |
RRID | AB_2199166 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis (PMID: 8058779). SYT1 (synaptotagmin I) was firstly identified as a 65-kDa protein with a wide distribution in neuronal and neurosecretory tissue (PMID: 7298720). Calcium binding to SYT1 participates in triggering neurotransmitter release at the synapse (PMID: 11242035). In addition to regulating exocytosis, SYT1 has also been implicated in endocytosis and neurite outgrowth (PMID: 15492212; 11078930; 10336114). This antibody recognizes endogenous SYT1, which has an experimentally determined molecular mass of 65-68 kDa. An additional band of 47 kDa could also be detected, which is corresponding to the calculated molecular weight of SYT1.
Protocols
Product Specific Protocols | |
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WB protocol for Synaptotagmin-1 antibody 14511-1-AP | Download protocol |
IHC protocol for Synaptotagmin-1 antibody 14511-1-AP | Download protocol |
IF protocol for Synaptotagmin-1 antibody 14511-1-AP | Download protocol |
IP protocol for Synaptotagmin-1 antibody 14511-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cell Rep The complement inhibitor CD59 is required for GABAergic synaptic transmission in the dentate gyrus | ||
Theranostics ANKRD22, a novel tumor microenvironment-induced mitochondrial protein promotes metabolic reprogramming of colorectal cancer cells. | ||
Front Mol Neurosci A Stimulation Function of Synaptotagmin-1 in Ternary SNARE Complex Formation Dependent on Munc18 and Munc13. | ||
Biochim Biophys Acta Mol Cell Biol Lipids Hepatic Synaptotagmin 1 is involved in the remodelling of liver plasma- membrane lipid composition and gene expression in male Apoe-deficient mice consuming a Western diet. | ||
J Proteomics Quantitative proteomics reveals olfactory input-dependent alterations in the mouse olfactory bulb proteome. | ||
PLoS One Identification of Protein Network Alterations upon Retinal Ischemia-Reperfusion Injury by Quantitative Proteomics Using a Rattus norvegicus Model. |