Syntaxin 1A / Syntaxin 1B Monoclonal antibody

• Featured Product
• KD/KO Validated

Syntaxin 1A / Syntaxin 1B Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, rabbit, pig

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Publications

9

CloneNo.

2D3B5

Cat no : 66437-1-Ig

Synonyms

STX1B, STX1B1, STX1B2, Syntaxin 1A / Syntaxin 1B, syntaxin 1B, Syntaxin 1B1, Syntaxin 1B2

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Validation Data Gallery

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  WB analysis using 66437-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of Y79 using 66437-1-Ig

  Y79 cells were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 66437-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of fetal human brain using 66437-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 66437-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 66437-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66437-1-Ig (Syntaxin 1A / Syntaxin 1B antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66437-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 66437-1-Ig

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66437-1-Ig (Syntaxin 1 Antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 66437-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Syntaxin 1A / Syntaxin 1B antibody (66437-1-Ig, Clone: 2D3B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: pig brain tissue, rat brain tissue, fetal human brain tissue, Y79 cells, rabbit brain tissue, mouse brain tissue
• Positive IHC detected in: rat brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:40000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 2 publications below

Product Information

66437-1-Ig targets Syntaxin 1A / Syntaxin 1B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, rabbit, pig samples.

• Tested Reactivity: human, mouse, rat, rabbit, pig
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Syntaxin 1A / Syntaxin 1B fusion protein Ag10257
• Full Name: syntaxin 1B
• Calculated Molecular Weight: 288 aa, 33 kDa
• Observed Molecular Weight: 33 kDa, 55 kDa
• GenBank Accession Number: BC062298
• Gene Symbol: STX1B
• Gene ID (NCBI): 112755
• RRID: AB_2881807
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody raised against 1-263 aa of human syntaxin 1B detects a ~33 kDa band of syntaxin 1 and an additional band of 55-60 kDa, which probably represents a syntaxin 1 dimer (PMID: 10100611).

Publications

Species	Application	Title
mouse	WB,IF	Nat Commun; Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.; Authors - I-Ling Lu ; KD Validated
rat	WB	J Neuroinflammation; Transient neuroinflammation following surgery contributes to long-lasting cognitive decline in elderly rats via dysfunction of synaptic NMDA receptor.; Authors - Bo Chen
mouse	WB	Clin Sci (Lond); Chronic hepatitis C virus infection impairs insulin secretion by regulation of p38δ MAPK-dependent exocytosis in pancreatic β-cells.; Authors - Jizheng Chen
human	WB,IF	Brain Commun; Transcriptomic analysis of human brains with Alzheimer's disease reveals the altered expression of synaptic genes linked to cognitive deficits.; Authors - Jamal B Williams
rat	WB	Nat Commun; Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly.; Authors - Shen Wang
mouse	WB	Neurotoxicology; Heterozygous disruption of beclin 1 alleviates neurotoxicity induced by sub-chronic exposure of arsenite in mice; Authors - Danyang Li