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Syntaxin 1A / Syntaxin 1B Monoclonal antibody
Syntaxin 1A / Syntaxin 1B Monoclonal Antibody for IF, IHC, WB, ELISA
Cat no : 66437-1-Ig
Validation Data Gallery
|Positive WB detected in||pig brain tissue, rat brain tissue, fetal human brain tissue, Y79 cells, rabbit brain tissue, mouse brain tissue|
|Positive IHC detected in||rat brain tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||mouse brain tissue|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunohistochemistry (IHC)||IHC : 1:2000-1:8000|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
66437-1-Ig targets Syntaxin 1A / Syntaxin 1B in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Mouse / IgG1|
|Immunogen||Syntaxin 1A / Syntaxin 1B fusion protein Ag10257|
|Full Name||syntaxin 1B|
|Calculated molecular weight||288 aa, 33 kDa|
|Observed molecular weight||33 kDa, 55 kDa|
|GenBank accession number||BC062298|
|Gene ID (NCBI)||112755|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Syntaxins are a family of transmembrane proteins that belong to the SNARE complex (PMID: 11737951). In conjunction with other SNAREs and with the cytoplasmic NSF and SNAP proteins, syntaxins mediate vesicle fusion in diverse vesicular transport processes along the exocytic and the endocytic pathway (PMID: 11737951). Syntaxin 1A and 1B, two closely related isoforms of syntaxin 1, are involved in synaptic vesicle docking and fusion during neurotransmitter release (PMID: 7690687; 1321498; 18691641). This antibody raised against 1-263 aa of human syntaxin 1B detects a ~33 kDa band of syntaxin 1 and an additional band of 55-60 kDa, which probably represents a syntaxin 1 dimer (PMID: 10100611).
|Product Specific Protocols|
|WB protocol for Syntaxin 1A / Syntaxin 1B antibody 66437-1-Ig||Download protocol|
|IHC protocol for Syntaxin 1A / Syntaxin 1B antibody 66437-1-Ig||Download protocol|
|IF protocol for Syntaxin 1A / Syntaxin 1B antibody 66437-1-Ig||Download protocol|
|Click here to view our Standard Protocols|
Identification of genes associated with cortical malformation using a transposon-mediated somatic mutagenesis screen in mice.
Transient neuroinflammation following surgery contributes to long-lasting cognitive decline in elderly rats via dysfunction of synaptic NMDA receptor.
Clin Sci (Lond)
Chronic hepatitis C virus infection impairs insulin secretion by regulation of p38δ MAPK-dependent exocytosis in pancreatic β-cells.
Transcriptomic analysis of human brains with Alzheimer's disease reveals the altered expression of synaptic genes linked to cognitive deficits.
Munc18 and Munc13 serve as a functional template to orchestrate neuronal SNARE complex assembly.
Heterozygous disruption of beclin 1 alleviates neurotoxicity induced by sub-chronic exposure of arsenite in mice