SMN Polyklonaler Antikörper

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• KD/KO Validated

SMN Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

14

Rezensionen (1)

Kat-Nr. : 11708-1-AP

Synonyme

SMN1,SMN, SMN1, Gemin-1, Gemin 1, Component of gems 1

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 11708-1-AP

  WB result of SMN antibody (11708-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SMN transfected HeLa cells.

• 

  WB analysis using 11708-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11708-1-PBS

  K-562 cells were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11708-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11708-1-AP (SMN antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 11708-1-AP

  IP result of anti-SMN (IP:11708-1-AP, 4ug; Detection:11708-1-AP 1:2000) with HEK-293 cells lysate 1040 ug.

• 

  IHC staining of human kidney using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 11708-1-AP (SMN antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 11708-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 11708-1-AP (SMN antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 11708-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SMN antibody (11708-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Maushodengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Herzgewebe, humanes Hirngewebe, humanes Hodengewebe, humanes Lungengewebe, humanes Milzgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• ELISA: See 4 publications below

Produktinformation

11708-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA SMN und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: SMN fusion protein Ag2260
• Vollständiger Name: survival of motor neuron 2, centromeric
• Berechnetes Molekulargewicht: 282 aa, 30 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC000908
• Gene symbol: SMN
• Gene ID (NCBI): 6607
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Spinal muscular atrophy (SMA) is an autosomal recessive neurodegenerative disease characterized by loss of anterior horn cells in the spinal cord and concomitant symmetrical muscle weakness and atrophy (PMID: 16364894 ). SMA is caused by deletion or mutations of the survival motor neuron (SMN1) gene. SMA patients lack a functional SMN1 gene, but they possess an intact SMN2 gene, which though nearly identical to SMN1, is only partially functional (PMID: 17355180). A large majority of SMN2 transcripts lack exon 7, resulting in production of a truncated, less stable SMN protein (PMID: 10369862). The level of SMN protein correlates with phenotypic severity of SMA. This antibody, 11708-1-AP, raised against the recombinant full-length human SMN2 protein, recognizes all isoforms of SMN protein.

Publikationen

Species	Application	Title
human	IF	Nat Commun; SNUPN deficiency causes a recessive muscular dystrophy due to RNA mis-splicing and ECM dysregulation; Authors - Marwan Nashabat
mouse	WB	Dev Cell; DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice; Authors - Dingfeng Zou
mouse	ELISA	Hum Mol Genet; SMN expression is required in motor neurons to rescue electrophysiological deficits in the SMNΔ7 mouse model of SMA.; Authors - Vicki L McGovern
human	WB	Hum Mol Genet; Low levels of Survival Motor Neuron protein are sufficient for normal muscle function in the SMNΔ7 mouse model of SMA.; Authors - Chitra C Iyer
mouse	ELISA	Neurobiol Dis; Dual SMN inducing therapies can rescue survival and motor unit function in symptomatic ∆7SMA mice.; Authors - Kaitlyn M Kray
human,mouse	WB	Hum Mol Genet; Intragenic complementation of amino and carboxy terminal SMN missense mutations can rescue Smn null mice.; Authors - Vicki L McGovern ; KO Validated

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Rachel (Verified Customer) (08-19-2019)

Worked well in MSD immunoassays and cell based assays.

• Applications: Western Blot, Cell culture,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fibroblasts

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