RTN3 Polyklonaler Antikörper
RTN3 Polyklonal Antikörper für WB, IP, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human und mehr (3)
Anwendung
WB, IP, IF, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 12055-2-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HEK-293T-Zellen, HeLa-Zellen, humanes Hirngewebe, U2OS-Zellen, U-87 MG-Zellen |
| Erfolgreiche IP | HeLa-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Veröffentlichte Anwendungen
| WB | See 16 publications below |
| IF | See 5 publications below |
| IP | See 2 publications below |
Produktinformation
12055-2-AP bindet in WB, IP, IF, ELISA RTN3 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| In Publikationen genannte Reaktivität | human, Affe, Maus, Ratte |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Polyklonal |
| Typ | Antikörper |
| Immunogen | RTN3 fusion protein Ag2685 |
| Vollständiger Name | reticulon 3 |
| Berechnetes Molekulargewicht | 113 kDa |
| Beobachtetes Molekulargewicht | 24 kDa, 100-113 kDa |
| GenBank-Zugangsnummer | BC011394 |
| Gene symbol | RTN3 |
| Gene ID (NCBI) | 10313 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Antigen-Affinitätsreinigung |
| Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
Reticulon (RTN) proteins are a group of membrane-bound proteins that largely reside in endoplasmic reticulum (ER). Reticulon proteins share a common sequence feature, the reticulon homology domain (RHD). Four mammalian reticulons (RTN1-4) exist. Reticulon3 (RTN3) is highly expressed in neurons. RTN3 has been shown to modulate secretory pathway protein trafficking. It is a negative modulator of BACE1 (β-secretase) proteolytic activity. RTN3 may induce caspase-8 cascade and apoptosis and may favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress.
Protokolle
| Produktspezifische Protokolle | |
|---|---|
| WB protocol for RTN3 antibody 12055-2-AP | Protokoll herunterladen |
| IP protocol for RTN3 antibody 12055-2-AP | Protokoll herunterladen |
| Standard-Protokolle | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
| Species | Application | Title |
|---|---|---|
Protein Cell Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice | ||
Acta Neuropathol Integrative proteomics highlight presynaptic alterations and c-Jun misactivation as convergent pathomechanisms in ALS | ||
J Pineal Res Melatonin restores endoplasmic reticulum homeostasis to protect injured neurons in a rat model of chronic cervical cord compression | ||
Dev Cell TAOK2 is an ER-localized kinase that catalyzes the dynamic tethering of ER to microtubules. | ||
Acta Neuropathol Commun Liver X receptor-agonist treatment rescues degeneration in a Drosophila model of hereditary spastic paraplegia. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Chun (Verified Customer) (09-07-2020) | This is a very good antibody for immunoblotting.
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