MED15 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MED15 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Rezensionen (1)

Kat-Nr. : 11566-1-AP

Synonyme

Mediator of RNA polymerase II transcription subunit 15, CTG7A, CTG repeat protein 7a, CAG7A, ARC105

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Galerie der Validierungsdaten

• 

  WB analysis of K-562 using 11566-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 11566-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HuH-7 using 11566-1-AP

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 11566-1-AP (MED15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 11566-1-AP

  IP result of anti-MED15 (IP:11566-1-AP, 3ug; Detection:11566-1-AP 1:500) with K-562 cells lysate 3440ug.

• 

  IHC staining of human prostate cancer using 11566-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11566-1-AP (MED15 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of SH-SY5Y using 11566-1-AP

  Immunofluorescent analysis of SH-SY5Y cells using 11566-1-AP (MED15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, SMMC-7721-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Prostatakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: SH-SY5Y-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 10 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• ChIP: See 4 publications below

Produktinformation

11566-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA MED15 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MED15 fusion protein Ag2143
• Vollständiger Name: mediator complex subunit 15
• Berechnetes Molekulargewicht: 788 aa, 87 kDa
• Beobachtetes Molekulargewicht: 105 kDa
• GenBank-Zugangsnummer: BC017110
• Gene symbol: MED15
• Gene ID (NCBI): 51586
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).

Publikationen

Species	Application	Title
		Mol Cell; Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals.; Authors - Nilay Shah
mouse	WB	Cell Rep; Mediator MED23 Links Pigmentation and DNA Repair through the Transcription Factor MITF.; Authors - Min Xia
human	WB,IHC,IF	Int J Cancer; MED15, encoding a subunit of the mediator complex, is overexpressed at high frequency in castration-resistant prostate cancer.; Authors - Zaki Shaikhibrahim ; KD Validated
human, mouse	ChIP	PLoS Genet; Mediator subunit MDT-15/MED15 and Nuclear Receptor HIZR-1/HNF4 cooperate to regulate toxic metal stress responses in Caenorhabditis elegans.; Authors - Naomi Shomer
human	WB	J Biol Chem; Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune-response genes.; Authors - Tsutsui Taiki T
human	WB, IHC, IF	Am J Pathol; Clinical and Molecular Implications of MED15 in Head and Neck Squamous Cell Carcinoma.; Authors - Zaki Shaikhibrahim

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

SITING (Verified Customer) (04-02-2021)

between 75-100

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: EMBRYO HEART

MED15 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:11566-1-AP)

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