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  • KD/KO Validated

MED15 Polyklonaler Antikörper

MED15 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11566-1-AP

Synonyme

Mediator of RNA polymerase II transcription subunit 15, CTG7A, CTG repeat protein 7a, CAG7A, ARC105



Geprüfte Anwendungen

Erfolgreiche Detektion in WBK-562-Zellen, SMMC-7721-Zellen
Erfolgreiche IPK-562-Zellen
Erfolgreiche Detektion in IHChumanes Prostatakarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
Erfolgreiche Detektion in IF/ICCSH-SY5Y-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:20-1:200
Immunfluoreszenz (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11566-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA MED15 und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MED15 fusion protein Ag2143
Vollständiger Name mediator complex subunit 15
Berechnetes Molekulargewicht 788 aa, 87 kDa
Beobachtetes Molekulargewicht 105 kDa
GenBank-ZugangsnummerBC017110
Gene symbol MED15
Gene ID (NCBI) 51586
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MED15, also named as TIG1, CAG7A, CTG7A, PCQAP, TIG-1, TNRC7 and ARC105, is a subunit of the multiprotein complexes PC2 and ARC/DRIP and may function as a transcriptional coactivator in RNA polymerase II transcription. It contains stretches of trinucleotide repeats and is located in the chromosome 22 region which is deleted in DiGeorge syndrome.MED15 is recruited to promoters by direct interaction with gene-specific regulatory proteins. It is about 105kd in SDS-page(doi.org/10.3109/10409238.2013.840259).

Protokolle

Produktspezifische Protokolle
WB protocol for MED15 antibody 11566-1-APProtokoll herunterladen
IHC protocol for MED15 antibody 11566-1-APProtokoll herunterladen
IF protocol for MED15 antibody 11566-1-APProtokoll herunterladen
IP protocol for MED15 antibody 11566-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle

Mol Cell

Tyrosine-1 of RNA Polymerase II CTD Controls Global Termination of Gene Transcription in Mammals.

Authors - Nilay Shah
mouseWB

Cell Rep

Mediator MED23 Links Pigmentation and DNA Repair through the Transcription Factor MITF.

Authors - Min Xia
humanWB,IHC,IF

Int J Cancer

MED15, encoding a subunit of the mediator complex, is overexpressed at high frequency in castration-resistant prostate cancer.

Authors - Zaki Shaikhibrahim
  • KD Validated
human, mouseChIP

PLoS Genet

Mediator subunit MDT-15/MED15 and Nuclear Receptor HIZR-1/HNF4 cooperate to regulate toxic metal stress responses in Caenorhabditis elegans.

Authors - Naomi Shomer
humanWB

J Biol Chem

Mediator complex recruits epigenetic regulators via its two cyclin-dependent kinase subunits to repress transcription of immune-response genes.

Authors - Tsutsui Taiki T
humanWB, IHC, IF

Am J Pathol

Clinical and Molecular Implications of MED15 in Head and Neck Squamous Cell Carcinoma.

Authors - Zaki Shaikhibrahim

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

SITING (Verified Customer) (04-02-2021)

between 75-100

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: EMBRYO HEART
MED15 Antibody Western Blot validation (1:500 dilution) in EMBRYO HEART (Cat no:11566-1-AP)