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MAP4 Polyklonaler Antikörper
MAP4 Polyklonal Antikörper für WB, IP, IHC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus, Ratte
Anwendung
WB, IP, IF, IHC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 11229-1-AP
Synonyme
Galerie der Validierungsdaten
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HeLa-Zellen, C2C12-Zellen, C6-Zellen, HEK-293-Zellen, HepG2-Zellen |
Erfolgreiche IP | HEK-293-Zellen |
Erfolgreiche Detektion in IHC | humanes Ösophaguskarzinomgewebe Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen. |
Empfohlene Verdünnung
Anwendung | Verdünnung |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunpräzipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunhistochemie (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Veröffentlichte Anwendungen
KD/KO | See 4 publications below |
WB | See 12 publications below |
IHC | See 6 publications below |
IF | See 2 publications below |
IP | See 1 publications below |
Produktinformation
11229-1-AP bindet in WB, IP, IF, IHC, ELISA MAP4 und zeigt Reaktivität mit human, Maus, Ratten
Getestete Reaktivität | human, Maus, Ratte |
In Publikationen genannte Reaktivität | human, Maus, Ratte |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | MAP4 fusion protein Ag1741 |
Vollständiger Name | microtubule-associated protein 4 |
Berechnetes Molekulargewicht | 121 kDa |
Beobachtetes Molekulargewicht | 210-240 kDa |
GenBank-Zugangsnummer | BC012794 |
Gene symbol | MAP4 |
Gene ID (NCBI) | 4134 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3. |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)
Protokolle
Produktspezifische Protokolle | |
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WB protocol for MAP4 antibody 11229-1-AP | Protokoll herunterladen |
IHC protocol for MAP4 antibody 11229-1-AP | Protokoll herunterladen |
IP protocol for MAP4 antibody 11229-1-AP | Protokoll herunterladen |
Standard-Protokolle | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Publikationen
Species | Application | Title |
---|---|---|
Acta Neuropathol Divergent brain gene expression patterns associate with distinct cell-specific tau neuropathology traits in progressive supranuclear palsy. | ||
Brain Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA. | ||
J Mol Cell Biol The molecular chaperone Hsp90 maintains Golgi organization and vesicular trafficking by regulating microtubule stability. | ||
Oncogene Microtubule-associated protein 4 is an important regulator of cell invasion/migration and a potential therapeutic target in esophageal squamous cell carcinoma.
| ||
J Innate Immun Ste20-Like Kinase TAOK1 Positively Regulates Antiviral Responses by Controlling the TBK1-IRF3 Signaling Axis | ||
Front Cell Infect Microbiol Rabies Virus Infection Induces Microtubule Depolymerization to Facilitate Viral RNA Synthesis by Upregulating HDAC6. |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alice (Verified Customer) (08-09-2022) | Not bad for western blot. A bit faint but had bands in expected places and can see clear data.
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FH CHAO (Verified Customer) (03-31-2022) | Multiple bands may be due to different post-translational modifications
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