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MAP4 Polyklonaler Antikörper

MAP4 Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 11229-1-AP

Synonyme

MAP 4, MAP4



Geprüfte Anwendungen

Erfolgreiche Detektion in WBHeLa-Zellen, C2C12-Zellen, C6-Zellen, HEK-293-Zellen, HepG2-Zellen
Erfolgreiche IPHEK-293-Zellen
Erfolgreiche Detektion in IHChumanes Ösophaguskarzinomgewebe
Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:3000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunhistochemie (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

11229-1-AP bindet in WB, IP, IF, IHC, ELISA MAP4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
In Publikationen genannte Reaktivitäthuman, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen MAP4 fusion protein Ag1741
Vollständiger Name microtubule-associated protein 4
Berechnetes Molekulargewicht 121 kDa
Beobachtetes Molekulargewicht 210-240 kDa
GenBank-ZugangsnummerBC012794
Gene symbol MAP4
Gene ID (NCBI) 4134
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAP4 is a ubiquitously expressed microtubule-associated protein involved in the organization and stabilization of microtubules during various cellular activities. Recently it has been reported that expression of MAP4 was upregulated in esophageal squamous cell carcinoma (ESCC). MAP4 has been considered as an independent prognostic factor for ESCC. The predicted molecular weight of MAP4 is about 120 kDa, while higher molecular weight around 200-250 kDa is usually observed in WB test, which may be the result of glycosylation. (26876215, 8647865)

Protokolle

Produktspezifische Protokolle
WB protocol for MAP4 antibody 11229-1-APProtokoll herunterladen
IHC protocol for MAP4 antibody 11229-1-APProtokoll herunterladen
IP protocol for MAP4 antibody 11229-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanIHC

Acta Neuropathol

Divergent brain gene expression patterns associate with distinct cell-specific tau neuropathology traits in progressive supranuclear palsy.

Authors - Mariet Allen
humanWB

Brain

Interactome screening of C9orf72 dipeptide repeats reveals VCP sequestration and functional impairment by polyGA.

Authors - Janja Božič
humanWB,IF

J Mol Cell Biol

The molecular chaperone Hsp90 maintains Golgi organization and vesicular trafficking by regulating microtubule stability.

Authors - Yuan Wu
humanWB

Oncogene

Microtubule-associated protein 4 is an important regulator of cell invasion/migration and a potential therapeutic target in esophageal squamous cell carcinoma.

Authors - Y-Y Jiang
  • KO Validated
mouseWB

J Innate Immun

Ste20-Like Kinase TAOK1 Positively Regulates Antiviral Responses by Controlling the TBK1-IRF3 Signaling Axis

Authors - Xiaogang Luo
humanWB

Front Cell Infect Microbiol

Rabies Virus Infection Induces Microtubule Depolymerization to Facilitate Viral RNA Synthesis by Upregulating HDAC6.

Authors - Jie Zan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Alice (Verified Customer) (08-09-2022)

Not bad for western blot. A bit faint but had bands in expected places and can see clear data.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Sheep atrial tissue
MAP4 Antibody Western Blot validation (1:1000 dilution) in Sheep atrial tissue (Cat no:11229-1-AP)
FH

CHAO (Verified Customer) (03-31-2022)

Multiple bands may be due to different post-translational modifications

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart