MAGOH Polyklonaler Antikörper

MAGOH Polyklonal Antikörper für WB, IP, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

9

Kat-Nr. : 12347-1-AP

Synonyme

MAGOH, MAGOHA, Protein mago nashi homolog

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Galerie der Validierungsdaten

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  WB analysis of K-562 using 12347-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 12347-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12347-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12347-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 12347-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 12347-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 12347-1-AP (MAGOH antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 12347-1-AP

  IP result of anti-MAGOH (IP:12347-1-AP, 3ug; Detection:12347-1-AP 1:500) with K-562 cells lysate 2400ug.

• 

  IHC staining of human ovary tumor using 12347-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12347-1-AP (MAGOH antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 12347-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12347-1-AP (MAGOH antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen, HL-60-Zellen, humanes Hirngewebe, Raji-Zellen
• Erfolgreiche IP: K-562-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Produktinformation

12347-1-AP bindet in WB, IP, IF, IHC, ELISA MAGOH und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MAGOH fusion protein Ag3004
• Vollständiger Name: mago-nashi homolog, proliferation-associated (Drosophila)
• Berechnetes Molekulargewicht: 146 aa, 17 kDa
• Beobachtetes Molekulargewicht: 17 kDa
• GenBank-Zugangsnummer: BC018211
• Gene symbol: MAGOH
• Gene ID (NCBI): 4116
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

MAGOH, belonging to the mago nashi family, is a component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Core components of the EJC functions to mark the position of the exon-exon junction in the mature mRNA and thereby influences downstream processes of gene expression including mRNA splicing, nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). MAGOH regulates the transcriptional activation of STAT3 by interfering complex formation between STAT3 and a core EJC component Y14.

Publikationen

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	IHC	Nat Neurosci; The exon junction complex component Magoh controls brain size by regulating neural stem cell division.; Authors - Silver Debra L DL
mouse	IHC	PLoS Genet; Haploinsufficiency for Core Exon Junction Complex Components Disrupts Embryonic Neurogenesis and Causes p53-Mediated Microcephaly.; Authors - Hanqian Mao
mouse	IF	Development; Dosage-dependent requirements of Magoh for cortical interneuron generation and survival.; Authors - Charles J Sheehan
mouse	WB	Genesis; Generation of a Magoh conditional allele in mice.; Authors - John J McMahon
human,mouse	WB,IHC,IP	J Exp Zool B Mol Dev Evol; Mago, a vertebrate homolog of Drosophila Mago nashi protein, is a component of the chromatoid body in the cytoplasm of the postmeiotic spermatid.; Authors - Zhao Wei W

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