UPF3B Polyclonal antibody

UPF3B Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 23301-1-AP

Synonyms

RENT3B, UPF3X, MRXS14, hUpf3p-X, hUpf3p X

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Validation Data Gallery

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  WB analysis using 23301-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23301-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 23301-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 23301-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23301-1-AP (UPF3B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary tumor using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23301-1-AP (UPF3B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 23301-1-AP (UPF3B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human heart using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 23301-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23301-1-AP (UPF3B Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, K-562 cells, Jurkat cells, HeLa cells
• Positive IHC detected in: human ovary tumor tissue, human skeletal muscle tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

23301-1-AP targets UPF3B in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UPF3B fusion protein Ag18568
• Full Name: UPF3 regulator of nonsense transcripts homolog B (yeast)
• Calculated Molecular Weight: 483 aa, 58 kDa
• Observed Molecular Weight: 58 kDa
• GenBank Accession Number: BC121017
• Gene Symbol: UPF3B
• Gene ID (NCBI): 65109
• RRID: AB_2879249
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Front Genet; Molecular Characterization and Clinical Relevance of RNA Binding Proteins in Colorectal Cancer.; Authors - Zhen Zhang