RBM8A,Y14 Polyclonal antibody

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  WB analysis using 14958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 14958-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14958-1-AP

  WB result of RBM8A,Y14 antibody (14958-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

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  WB analysis of HeLa using 14958-1-AP

  WB result of RBM8A,Y14 antibody (14958-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM8A,Y14 transfected HeLa cells.

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  WB analysis of Jurkat using 14958-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14958-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14958-1-AP (RBM8A,Y14 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IP experiment of HeLa using 14958-1-AP

  IP result of anti-RBM8A,Y14 (IP:14958-1-AP, 4ug; Detection:14958-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human brain using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 14958-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of HeLa using 14958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM8A,Y14 antibody (14958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 14958-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RBM8A,Y14 antibody (14958-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of HeLa using 14958-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 14958-1-AP (RBM8A,Y14 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

RBM8A,Y14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14958-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RBM8A, BOV-1, BOV 1C, BOV 1B, BOV 1A

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Tested Applications

• Positive WB detected in: HEK-293T cells, Jurkat cells, HeLa cells, MDA-MB-453s cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human brain tissue, human spleen tissue, human testis tissue, human ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

14958-1-AP targets RBM8A,Y14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RBM8A,Y14 fusion protein Ag6800
• Full Name: RNA binding motif protein 8A
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC017088
• Gene Symbol: RBM8A
• Gene ID (NCBI): 9939
• RRID: AB_2178820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5S9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Cell Metab; NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis.; Authors - Mi Kyung Park
mouse	IF	RNA; An atlas of chromatoid body components.; Authors - Oliver Meikar
human	WB,IHC	Front Oncol; RBM8A Promotes Glioblastoma Growth and Invasion Through the Notch/STAT3 Pathway.; Authors - Yan Lin ; KD Validated
human	WB	Cell Death Discov; CircEIF3H-IGF2BP2-HuR scaffold complex promotes TNBC progression via stabilizing HSPD1/RBM8A/G3BP1 mRNA.; Authors - Xiaojin Song
human	WB,IF	Cell Rep; The RNA-binding protein EIF4A3 promotes axon development by direct control of the cytoskeleton; Authors - Fernando C Alsina