NXF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

NXF1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 10328-1-AP

Synonyms

Tip-associated protein, Tip associating protein, Tip associated protein, TAP, mRNA export factor TAP

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Validation Data Gallery

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  WB analysis of HeLa using 10328-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10328-1-AP

  WB result of NXF1 antibody (10328-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NXF1 transfected HeLa cells.

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  WB analysis using 10328-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 10328-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10328-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10328-1-AP (NXF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10328-1-AP

  IP result of anti-NXF1 (IP:10328-1-AP, 4ug; Detection:10328-1-AP 1:500) with HeLa cells lysate 3000 ug.

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  IF Staining of HepG2 using 10328-1-AP

  Immunofluorescent analysis of HepG2 cells using 10328-1-AP (NXF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, K-562 cells, HepG2 cells
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

10328-1-AP targets NXF1 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NXF1 fusion protein Ag0413
• Full Name: nuclear RNA export factor 1
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC004904
• Gene Symbol: NXF1
• Gene ID (NCBI): 10482
• RRID: AB_2236500
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear RNA export factor 1 (NXF1, synonyms: TAP, MEX67) is one member of a family of nuclear RNA export factors. Common domain features of this family are a noncanonical RNP-type RNA-binding domain (RBD), 4 leucine-rich repeats (LRRs), a nuclear transport factor 2 (NTF2)-like domain that allows heterodimerization with NTF2-related export protein-1(NXT1), and a ubiquitin-associated domain that mediates interactions with nucleoporins. The LRRs and NTF2-like domains are required for export activity. This protein shuttles between the nucleus and the cytoplasm and binds in vivo to poly(A)+ RNA. It is the vertebrate homologue of the yeast protein Mex67p. NXF1 overcomes the mRNA export block caused by the presence of saturating amounts of CTE (constitutive transport element) RNA of type D retroviruses. There are some phosphorylation modification sites center on the N-terminal of NXF1.

Publications

Species	Application	Title
human	WB	J Cell Biol; Inhibition of pyrimidine synthesis reverses viral virulence factor-mediated block of mRNA nuclear export.; Authors - Zhang Liang L ; KD Validated
		J Cell Biol; Intronless mRNAs transit through nuclear speckles to gain export competence.; Authors - Ke Wang
human	WB	Nucleic Acids Res; Depletion of REF/Aly alters gene expression and reduces RNA polymerase II occupancy.; Authors - Sarah H Stubbs
human	WB	PLoS Genet; Nucleoporin TPR promotes tRNA nuclear export and protein synthesis in lung cancer cells.; Authors - Miao Chen ; KD Validated
monkey	WB,CoIP	Mol Cell Biol; Fragile X mental retardation protein FMRP binds mRNAs in the nucleus.; Authors - Kim Miri M ; KD Validated
human	WB,IF,IP	Chromosoma; XIST RNA exhibits nuclear retention and exhibits reduced association with the export factor TAP/NXF1.; Authors - Cohen Hannah R HR